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Study of the Adhesion of Bifidobacterium bifidum MIMBb75 to Human Intestinal Cell Lines

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Abstract

The aim of this study was to investigate the adhesive phenotype of the human intestinal isolate Bifidobacterium bifidum MIMBb75 to human colon carcinoma cell lines. We have previously shown that the adhesion of this strain to Caco-2 cells is mediated by an abundant surface lipoprotein named BopA. In this study, we found that this strain adheres to Caco-2 and HT-29 cells, and that its adhesion strongly depends on the environmental conditions, including the presence of sugars and bile salts and the pH. Considerably more adhesion to a Caco-2 monolayer occurred in the presence of fucose and mannose and less when MIMBb75 grew in Oxgall bile salts compared to standard environmental conditions. In particular, growth in Oxgall bile salts reduced the adhesion ability of MIMBb75 and modified the SDS-PAGE profile of the cell wall associated proteins of the strain. The pH markedly affected both adhesion to Caco-2 and bacterial autoaggregation. Finally, experiments with sodium metaperiodate suggested that not only proteinaceous determinants are involved in the adhesion process of B. bifidum. In conclusion, it seems that the colonization strategy of this bacterium can be influenced by factors varying along the gastrointestinal tract, such as the presence of specific sugars and bile salts and the pH, possibly limiting the adhesion of B. bifidum to only restricted distal sites of the gut.

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References

  1. Bibiloni R, Pérez PF, De Antoni GL (1999) Factors involved in adhesion of bifidobacterial strains to epithelial cells in culture. Anaerobe 5:483–485

    Article  CAS  Google Scholar 

  2. Conway PL, Kjelleberg S (1998) Protein-mediated adhesion of Lactobacillus fermentum strain 737 to mouse stomach squamous epithelium. J Gen Microbiol 135:1175–1186

    Google Scholar 

  3. Gómes-Zavaglia A, Kociubinski G, Perez P, Disalvo E, De Antoni G (2002) Effect of bile on the lipid composition and surface properties of bifidobacteria. J Appl Microbiol 93:794–799

    Article  Google Scholar 

  4. Granato D, Perotti F, Masserey I, Rouvet M, Golliard M, Servin A, Brassart D (1999) Cell surface-associated lipoteichoic acid acts as an adhesion factor for attachment of Lactobacillus johnsonii La1 to human enterocyte-like Caco-2 cells. Appl Environ Microbiol 65:1071–1077

    PubMed  CAS  Google Scholar 

  5. Greene JD, Klaenhammer TR (1994) Factors involved in adherence of lactobacilli to human Caco-2 cells. Appl Environ Microbiol 60:4487–4494

    PubMed  CAS  Google Scholar 

  6. Guglielmetti S, Tamagnini I, Mora D, Minuzzo M, Scarafoni A, Arioli S, Hellman J, Karp M, Parini C (2008) Implication of an outer surface lipoprotein in adhesion of Bifidobacterium bifidum to Caco-2 cells. Appl Environ Microbiol 74:4695–4702

    Article  PubMed  CAS  Google Scholar 

  7. He F, Ouwehand AC, Isolauri E, Hosoda M, Benno Y, Salminen S (2001) Differences in composition and mucosal adhesion of bifidobacteria isolated from healthy adults and healthy seniors. Curr Microbiol 43:351–354

    Article  PubMed  CAS  Google Scholar 

  8. Kirjavainen PV, Ouwehand AC, Isolauri E, Salminen SJ (1998) The ability of probiotic bacteria to bind to human intestinal mucus. FEMS Microbiol Lett 167:185–189

    Article  PubMed  CAS  Google Scholar 

  9. Kleeman EG, Klaenhammer TR (1982) Adherence of Lactobacillus species to human fetal intestinal cells. J Dairy Sci 65:2063–2069

    Article  PubMed  CAS  Google Scholar 

  10. Langendijk PS, Schut F, Jansen GJ, Raangs GC, Kamphuis GR, Wilkinson MH, Welling GW (1995) Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples. Appl Environ Microbiol 61:3069–3075

    PubMed  CAS  Google Scholar 

  11. Lee YK, Nomoto K, Salminen S, Gorgach SJ (1999) Handbook of probiotics. Wiley & Sons, New York

    Google Scholar 

  12. Lehto EM, Salminen SJ (1997) Inhibition of Salmonella typhimurium adhesion to Caco-2 cell cultures by Lactobacillus strain GG spent culture supernate: only a pH effect? FEMS Immunol Med Microbiol 18:125–132

    Article  PubMed  CAS  Google Scholar 

  13. Mattarelli P, Biavati B, Crociani F, Scardovi V, Prati G (1993) Bifidobacterial cell wall proteins (BIFOP) in Bifidobacterium globosum. Res Microbiol 144:581–590

    Article  PubMed  CAS  Google Scholar 

  14. Morita H, He F, Fuse T, Ouwehand AC, Hashimoto H, Hosoda M, Mizumachi K, Kurisaki J (2002) Adhesion of lactic acid bacteria to caco-2 cells and their effect on cytokine secretion. Microbiol Immunol 46:293–297

    PubMed  CAS  Google Scholar 

  15. Op den Camp HJ, Oosterhof A, Veerkamp JH (1985) Interaction of bifidobacterial lipoteichoic acid with human intestinal epithelial cells. Infect Immun 47:332–334

    PubMed  CAS  Google Scholar 

  16. Ouwehand AC, Tuomola EM, Tölkkö S, Salminen S (2001) Assessment of adhesion properties of novel probiotic strains to human intestinal mucus. Int J Food Microbiol 64:119–126

    Article  PubMed  CAS  Google Scholar 

  17. Perez PF, Minnaard Y, Disalvo EA, De Antoni GL (1998) Surface properties of bifidobacterial strains of human origin. Appl Environ Microbiol 64:21–26

    PubMed  CAS  Google Scholar 

  18. Rautava S, Walker WA (2007) Commensal bacteria and epithelial cross talk in the developing intestine. Curr Gastroenterol Rep 9:385–392

    Article  PubMed  Google Scholar 

  19. Riedel CU, Foata F, Goldstein DR, Blum S, Eikmanns BJ (2006) Interaction of bifidobacteria with Caco-2 cells-adhesion and impact on expression profiles. Int J Food Microbiol 110:62–68

    Article  PubMed  CAS  Google Scholar 

  20. Rousset M (1986) The human colon carcinoma cell lines HT-29 and Caco-2: two in vitro models for the study of intestinal differentiation. Biochimie 68:1035–1040

    Article  PubMed  CAS  Google Scholar 

  21. Sonnenburg JL, Angenent LT, Gordon JI (2004) Getting a grip on things: how do communities of bacterial symbionts become established in our intestine? Nat Immunol 5:569–573

    Article  PubMed  CAS  Google Scholar 

  22. Vizoso Pinto MG, Schuster T, Briviba K, Watzl B, Holzapfel WH, Franz CM (2007) Adhesive and chemokine stimulatory properties of potentially probiotic Lactobacillus strains. J Food Prot 70:125–134

    PubMed  Google Scholar 

  23. Whittaker CJ, Klier CM, Kolenbrander PE (1996) Mechanisms of adhesion by oral bacteria. Annu Rev Microbiol 50:513–552

    Article  PubMed  CAS  Google Scholar 

  24. Youakim A, Herscovics A (1985) Cell surface glycopeptides from human intestinal epithelial cell lines derived from normal colon and colon adenocarcinomas. Cancer Res 45:5505–5511

    PubMed  CAS  Google Scholar 

  25. Zárate G, Morata De Ambrosini V, Perez Chaia A, Gonzalez S (2002) Some factors affecting the adherence of probiotic Propionibacterium acidipropionici CRL 1198 to intestinal epithelial cells. Can J Microbiol 48:449–457

    Article  PubMed  Google Scholar 

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Acknowledgment

This work has been partially funded by the Italian Government grant PUR 2008.

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Authors and Affiliations

  1. Department of Food Science and Microbiology, University of Milan, Milan, Italy

    Simone Guglielmetti, Isabella Tamagnini, Stefania Arioli, Carlo Parini & Diego Mora

  2. Department of Biomolecular Sciences and Biotechnology, University of Milan, Milan, Italy

    Mario Minuzzo

  3. Department of Nutritional Sciences, University of Toronto, Toronto, Canada

    Elena Comelli

Authors
  1. Simone Guglielmetti
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  2. Isabella Tamagnini
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  3. Mario Minuzzo
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  4. Stefania Arioli
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  5. Carlo Parini
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  6. Elena Comelli
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  7. Diego Mora
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Correspondence to Simone Guglielmetti.

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Guglielmetti, S., Tamagnini, I., Minuzzo, M. et al. Study of the Adhesion of Bifidobacterium bifidum MIMBb75 to Human Intestinal Cell Lines. Curr Microbiol 59, 167–172 (2009). https://doi.org/10.1007/s00284-009-9415-x

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  • DOI: https://doi.org/10.1007/s00284-009-9415-x

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