Abstract
Previously, a new Cordyceps species was isolated from a low altitude area in Chongqing, China, and named Cordyceps chongqingensis sp. nov. In this study, its anamorph was isolated and designated CQM1T. It grew optimally on Czapek medium supplied with 0.5% silkworm flour and 0.5% soybean oil meal at 25°C, pH 5.0–5.5. The phenotypic and molecular characteristics were investigated for its identification and typing. Morphological observations under a microscope revealed that this anamorph of Cordyceps chongqingensis sp. nov. was a new species of Metarhizium. Moreover, it was identified as one of the variants of Metarhizium taii based on sequences of 26S rDNA D1/D2 and ITS regions, and thus named Metarhizium taii var. chongqingensis nov.
Similar content being viewed by others
References
Kirk PM, Cannon PF, David JC, Stalpers JA (2001) Ainsworth & Bisby’s dictionary of the fungi, 9th edn. CAB International University Press, Cambridge, United Kingdom, p 655
Li CR, Fan MZ, Huang B, Wang SB, Li ZZ (2002) The genus Cordyceps and its allies from Anhui. Mycostema 21:167–171
Lin QY, Zhong YQ, Li TH, Shen YH, Song B (2006) Recent research advances in Cordyceps biology. Acta Edulis Fungi 13:93–98
Pu ZL, Li ZZ (1996) Entomomycology. Anhui Science & Technology Press, Heifei
Chen QT, Xiao SR, Shi ZY (1984) Paecilomyces sinensis sp. nov. and its connection with Cordyceps sinensis. Mycol Sin 3:24–28
Obornrik M, Jirku M, Dolezel D (2001) Phylogeny of mitosporic entomopathogenic fungi: is the genus Paecilomyces polyphyletic? Can J Microbiol 47:813–819
Liu XJ, Guo YL, Yu YX, Zeng W (1989) Isolation and identification of the anamorphic state of Cordyceps sinensis (Berk.) SACC. Mycol Sin 8:35–40
Liu ZY, Yao YJ, Liang ZQ (2001) Molecular evidence for the anamorph-teleomorph connection in Cordyceps sinensis. Mycol Res 105:827–832
Li ZZ, Huang B, Li CR, Fan MZ (2000) Molecular evidence for anamorph determination of Cordyceps Sinensis (Berk.) SACC.I. Relation between Hirsutella sinensis and C. sinensis. Mycosystema 19:60–64
Liang ZQ, Liu AY, Liu JL (1991) A new species of the genus Cordyceps and its Metarhizium anamorph. Acta Mycol Sin 10:257–262
Li ZL (1988) A study of Tolypocladium sinense C. L. Li. sp. nov. and cyclosporin production. Mycol Sin 7:93–98
Liang ZQ (1985) Isolation and identification of anamorph of Cordyceps gunnii. Acta Mycol Sin 4:162–166
Qian CR, Huang YX (1991) Microbiology experimental book. Beijing University Press, Beijing, p 45
Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416
Guo HL, Chen QT (1990) An emendation of Metarhizium (Metsch.) Sorokin. J Microbiol 12:7–10
Tulloch M (1976) The genus Metarhizium. Trans Br Mycol Soc 66:407–411
Chen YQ, Hu B, Xu F, Zhang WM, Zhou H, Qu LH (2004) Genetic variation of Cordyceps sinensis, a fruit-body-producing entomopathogenic species from different geographical regions in China. FEMS Microbiol Lett 230:153–158
Hattori M, Yokoyama E, Isomura S, Ujita M, Hara A (2006) Entomogenous fungi that produce 2,6-pyridine dicarboxylic acid (dipicolinic acid). J Biosci Bioeng 102:365–368
Kang JC, Liang ZQ, Liu AY, Richard YCK (2000) Molecular evidence of polymorphism in Cordycps based on 5.8S rDNA and ITS2 sequences. Mycosystema 19:492–497
Wang N, Chen YQ, Zhang WM, Li TH, Qu LH (2000) Molecular evidences indicating multiple origins in the entomogenous Cordyceps. Acta Sci Nat Univ Sunyatseni 39:71–74
Zhao J, Wang N, Chen YQ, Li TH, Qu LH (1999) Molecular identification for the asexual stage of Cordyceps sinensis. Acta Sci Nat Univ Sunyatseni 38:121–123
Acknowledgments
We wish to thank Shanquan Fu for his kind help with sampling. The research was funded by Science and Technology Research Fund Grant of Chongqing Municipal Education Commission, China (No. 2006CJ19-KJ060618). Molecular identification was supported by a PhD fellowship from The Third Military Medical University.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yang, Y., Cai, S., Zheng, Y. et al. Metarhizium taii var. chongqingensis Nov., Anamorph of Cordyceps chongqingensis sp. Nov. Isolated from a Low Altitude Area in Chongqing, China. Curr Microbiol 58, 635–641 (2009). https://doi.org/10.1007/s00284-009-9382-2
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00284-009-9382-2