To better understand how the reducing power of either NADH or NADPH affects cell growth, Escherichia coli strains expressing either NADH-dependent or NADPH-dependent azoreductase (EC 184.108.40.206), which mediates the reduction of an azo dye, were cultured in glucose minimal medium in the presence of 200 μM methyl red. Growth rates in NADH-perturbed, NADPH-perturbed, and control cells were 0.05, 0.12, and 0.13 h−1, respectively. In addition, glucose consumption in NADH-perturbed cells was 10.8 g glucose/g cell, while that of control and NADPH-perturbed cells was very similar (3.6 vs 3.8 g glucose/g cell) during the perturbation phase. Therefore, NADH perturbation had a larger effect than NADPH on cellular growth.
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The authors appreciate the financial support from the Korean Ministry of Education, Science and Technology (Basic Research Program of KOSEF) and Gyunggi Province (GRRC program to S. Kim, D. Moon, and P. Kim).
Susie Kim, Doo-Bum Moon contributed equally to this article.
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Kim, S., Moon, DB., Lee, CH. et al. Comparison of the Effects of NADH- and NADPH-Perturbation Stresses on the Growth of Escherichia coli . Curr Microbiol 58, 159–163 (2009). https://doi.org/10.1007/s00284-008-9294-6