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Comparison of the Effects of NADH- and NADPH-Perturbation Stresses on the Growth of Escherichia coli

Current Microbiology volume 58, pages 159–163 (2009)Cite this article

Abstract

To better understand how the reducing power of either NADH or NADPH affects cell growth, Escherichia coli strains expressing either NADH-dependent or NADPH-dependent azoreductase (EC 1.6.5.2), which mediates the reduction of an azo dye, were cultured in glucose minimal medium in the presence of 200 μM methyl red. Growth rates in NADH-perturbed, NADPH-perturbed, and control cells were 0.05, 0.12, and 0.13 h−1, respectively. In addition, glucose consumption in NADH-perturbed cells was 10.8 g glucose/g cell, while that of control and NADPH-perturbed cells was very similar (3.6 vs 3.8 g glucose/g cell) during the perturbation phase. Therefore, NADH perturbation had a larger effect than NADPH on cellular growth.

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Acknowledgments

The authors appreciate the financial support from the Korean Ministry of Education, Science and Technology (Basic Research Program of KOSEF) and Gyunggi Province (GRRC program to S. Kim, D. Moon, and P. Kim).

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Authors and Affiliations

  1. Department of Biotechnology, Catholic University of Korea, Bucheon, Gyunggi, 420-743, South Korea

    Susie Kim, Doo-Bum Moon & Pil Kim

  2. Department of Bioscience and Biotechnology, Konkuk University, Seoul, 143-701, South Korea

    Chung-Hwan Lee

  3. Department of Biomaterial Control, Dong-Eui University, Busan, 614-714, South Korea

    Soo-Wan Nam

Authors
  1. Susie Kim
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  2. Doo-Bum Moon
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  3. Chung-Hwan Lee
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  4. Soo-Wan Nam
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  5. Pil Kim
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Corresponding author

Correspondence to Pil Kim.

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Susie Kim, Doo-Bum Moon contributed equally to this article.

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Kim, S., Moon, DB., Lee, CH. et al. Comparison of the Effects of NADH- and NADPH-Perturbation Stresses on the Growth of Escherichia coli . Curr Microbiol 58, 159–163 (2009). https://doi.org/10.1007/s00284-008-9294-6

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  • DOI: https://doi.org/10.1007/s00284-008-9294-6

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