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Vegetative Incompatibility Among Monoconidial Isolates of Bipolaris sorokiniana

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Abstract

Bipolaris sorokiniana is a phytopathogenic fungus that causes diseases of cereal crops, such as leaf-spot disease, common root rot, and black point of grain. Because of its great morphological, physiological, and genetic variability, this fungus is difficult to control. The aim of this investigation was to study the variability of isolates of B. sorokiniana by means of vegetative incompatibility. Thirty-five isolates of B. sorokiniana from different geographical regions in Brazil and other countries were used. The vegetative incompatibility between the isolates and the influences of different culture media on these reactions were evaluated. The total protein profile of the isolates was analyzed when the isolates were cultured separately, and in cultures of compatibility and incompatibility reactions. Eighteen of 31 confrontations showed vegetative incompatibility. The results obtained with different culture media for the vegetative compatibility/incompatibility genotypes suggested that the type of substratum influences these reactions. No differences in protein profiles among the isolates were observed. This result suggests that there is no induction of expression of different proteins in vegetative incompatibility reactions.

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Acknowledgments

We are grateful to Dr. Julie Nicol, Dr. Monica Mezzalama, Dr. Etienne Duveiller, and Dr. Henri Maraite, of CIMMYT, and Dr. Kerry O′Donnell, of the USDA, for kindly providing the Bipolaris sorokiana samples from the other countries and to Dr. Airano Prestes, of EMBRAPA (Passo Fundo, Brazil), for providing the B. sorokiniana isolates from barley and the wheat seeds. This study was financed by CAPES/PROF.

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  1. Departamento de Microbiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

    A. Poloni, I. S. Pessi, A. P. G. Frazzon & S. T. Van Der Sand

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  1. A. Poloni
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  2. I. S. Pessi
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  3. A. P. G. Frazzon
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  4. S. T. Van Der Sand
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Correspondence to S. T. Van Der Sand.

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Poloni, A., Pessi, I.S., Frazzon, A.P.G. et al. Vegetative Incompatibility Among Monoconidial Isolates of Bipolaris sorokiniana . Curr Microbiol 58, 153–158 (2009). https://doi.org/10.1007/s00284-008-9292-8

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  • DOI: https://doi.org/10.1007/s00284-008-9292-8

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