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Transformation of L-Tyrosine to L-Dopa by a Novel Fungus, Acremonium rutilum, Under Submerged Fermentation

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Abstract

The present study deals with the transformation of L-tyrosine to L-dopa by Acremonium rutilum, a fungal tyrosinase producer, isolated from decomposed banana stud. This appears to be the first report on A. rutilum as a polyphenoloxidase producer with both cresolase and catecholase activity. Enriched Czapek-Dox agar was used for plate assay screening. Enriched potato dextrose broth was used for optimization studies, which induced high levels of L-dopa under submerged fermentation. A. rutilum gave the maximum L-dopa production (0.89 mg/ml) and tyrosinase activity (1095 U/mg) under the optimized parameters, that is, a temperature of 25°C, pH 5.5, an inoculum size of 2.5 ml, and an incubation time of 72–120 h, with L-tyrosine (5 mg/ml) as substrate. Five resolved bands, with Rf values of 0.73, 0.60, 0.54, 0.37, and 0.26, were observed, which confirmed the presence of L-dopa. This study involves the elevated profile of L-dopa production. Such study is needed, as L-dopa has the ability to control Parkinson’s disease.

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Acknowledgment

The first author is thankful to Gulbarga University, Gulbarga, for the award of a merit studentship and UGC for the award of Research Fellowship.

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Authors and Affiliations

  1. Department of Microbiology, Gulbarga University, Gulbarga, 585106, India

    R. Krishnaveni, Vandana Rathod & Y. F. Neelgund

  2. Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Mysore, 570020, India

    M. S. Thakur

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  1. R. Krishnaveni
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  2. Vandana Rathod
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  3. M. S. Thakur
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  4. Y. F. Neelgund
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Correspondence to Vandana Rathod.

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Krishnaveni, R., Rathod, V., Thakur, M.S. et al. Transformation of L-Tyrosine to L-Dopa by a Novel Fungus, Acremonium rutilum, Under Submerged Fermentation. Curr Microbiol 58, 122–128 (2009). https://doi.org/10.1007/s00284-008-9287-5

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  • DOI: https://doi.org/10.1007/s00284-008-9287-5

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