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Heterologous Expression of Oxalate Decarboxylase in Lactobacillus plantarum NC8

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Abstract

Lactic acid bacteria (LABs) are being used as a probiotic very often for various enteric problems. Many genetically modified LABs are created by different workers for various novel applications. In this study we examine the expression of heterologous oxalate decarboxylase (oxdc) in Lactobacillus plantarum NC8. Generally, this enzyme is not present in Lactobacillus spp. Oxdc gene from Bacillus subtilis was polymerase chain reaction-amplified and cloned in a shuttle vector pSIP400 series, downstream of the inducible promoter, Porfx. In the presence of an inducing peptide, Sakacin-P, the expression of OxdC was observed in sodium dodecyl sulfate–polyacrylamide gel electrophoresis. The cell-free extract and the purified protein from the recombinant LABs showed the presence of OxdC activity. The above recombinant LABs, with desired modifications, can be used as a possible probiotic for the degradation of intestinal dietary oxalate for preventing enteric hyperoxaluria.

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Acknowledgments

The first author acknowledges the Council for Scientific and Industrial Research (CSIR) for providing the senior research fellowship. The authors wish to thank Dr. Lars Axelsson for providing the plasmid pSIP409, induction peptide, and Lb.plantarum NC8. Further thanks to Dr. Stephen Bornemann for providing the plasmid pLB36 consisting oxdc gene.

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  1. Department of Biochemistry, School of Biological Sciences, Madurai Kamaraj University, Madurai, TN, 625021, India

    Anbazhagan Kolandaswamy, Leema George & Selvam Sadasivam

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  1. Anbazhagan Kolandaswamy
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  2. Leema George
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  3. Selvam Sadasivam
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Correspondence to Selvam Sadasivam.

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Kolandaswamy, A., George, L. & Sadasivam, S. Heterologous Expression of Oxalate Decarboxylase in Lactobacillus plantarum NC8. Curr Microbiol 58, 117–121 (2009). https://doi.org/10.1007/s00284-008-9286-6

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  • DOI: https://doi.org/10.1007/s00284-008-9286-6

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