Abstract
An anaerobic fungal isolate, CR4, was isolated from the bovine rumen. The DNA sequence of internal transcribed spacer region 1 showed that CR4 belonged to the genus Caecocmyces. The dry matter digestibility of timothy hay by anaerobic fungal isolate CR4 was determined. The effects of carbohydrate growth substrates on carboxymethyl cellulase (CMCase) and xylanase activities also were examined. The extent of dry matter digestibility of timothy hay was 31% at 6 days’ incubation. The highest specific activity of CMCase in the culture supernatant (SN) fraction was observed in xylose culture. The activity of CMCase was not detected in the SN fraction of cellobiose and xylan or in the cell-bound fraction of all growth substrates. The highest specific activity of xylanase in the SN fraction was observed in glucose culture. These results suggest that fiber-degrading enzyme activities were affected by growth substrates and that CR4 is xylanolytic. Zymogram analysis showed that CR4 produces three CMCases of molecular mass (95, 89, and 64 kDa) and three xylanases of molecular mass (82, 73, and 66 kDa). This is the first demonstration showing the molecular mass of fiber-degrading enzymes of Caecomyces.
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Acknowledgments
The authors would like to thank Dr. Yasunari Yamamoto (Livestock Research Division, Mie Prefectural Science and Technology Promotion Center) for providing rumen fluid. This study was supported by the Integrated Research for Developing Japanese-style Forage Feeding System to Increase Forage Self-Support Ratio of the Ministry of Agriculture, Forestry and Fisheries of Japan. The authors would also thank to Ms. Ayaka Fukuda (Mie University) for her assistance in cultivation and measurements. Nucleotide sequence analysis was done at the Life Science Research Center, Center for Molecular Biology, and Genetics of Mie University.
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Matsui, H., Ban-Tokuda, T. Studies on Carboxymethyl Cellulase and Xylanase Activities of Anaerobic Fungal Isolate CR4 from the Bovine Rumen. Curr Microbiol 57, 615–619 (2008). https://doi.org/10.1007/s00284-008-9252-3
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DOI: https://doi.org/10.1007/s00284-008-9252-3