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In Vitro Adsorption Revealing an Apparent Strong Interaction Between Endophyte Pantoea agglomerans YS19 and Host Rice

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Abstract

Pantoea (formerly Enterobacter) agglomerans YS19 is a dominant diazotrophic endophyte isolated from rice (Oryza sativa cv. Yuefu) grown in a temperate-climate region in west Beijing, China. In vitro adsorption and invasion of YS19 on host plant root were studied in this research. Adsorption of YS19 on rice seedling roots closely resembled the Langmuir adsorption and showed a higher adsorption quantity than the control strains Paenibacillus polymyxa WY110 (a rhizospheric bacterium from the same rice cultivar) and Escherichia coli HB101 (a general model bacterium). Adsorption dynamics study revealed high rates and a long duration of the YS19-rice root adsorption process. Adsorption of YS19 was mainly observed on the root hair, though which it enters the plant. This in vitro adsorption study revealed an apparent strong interaction between YS19 and rice at the early endophyte-host recognition stage.

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Acknowledgments

This work was supported by National Natural Science Foundation of China 30400002. The authors are grateful to Prof. T. Han, Mr. C. Wu, and Dr. W. Hou (Institute of Crop Sciences, Chinese Academy of Agricultural Sciences) for their constructive suggestions.

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Authors and Affiliations

  1. School of Life Science and Technology, Beijing Institute of Technology, 5# Zhongguankun Nondajie, 100081, Beijing, People’s Republic of China

    Yuxuan Miao, Jia Zhou, Cuicui Chen & Yongjun Feng

  2. Institute of Soil and Fertilizer, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

    Delong Shen

  3. College of Life Sciences, Capital Normal University, Beijing, People’s Republic of China

    Wei Song

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  1. Yuxuan Miao
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  2. Jia Zhou
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  3. Cuicui Chen
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  4. Delong Shen
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  5. Wei Song
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  6. Yongjun Feng
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Correspondence to Yongjun Feng.

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Miao, Y., Zhou, J., Chen, C. et al. In Vitro Adsorption Revealing an Apparent Strong Interaction Between Endophyte Pantoea agglomerans YS19 and Host Rice. Curr Microbiol 57, 547–551 (2008). https://doi.org/10.1007/s00284-008-9240-7

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  • DOI: https://doi.org/10.1007/s00284-008-9240-7

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