Isolation and Characterization of ACC Deaminase Gene from Two Plant Growth-Promoting Rhizobacteria
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Lowering of plant ethylene by deamination of its immediate precursor 1-aminocyclopropane-1-carboxylate (ACC) is a key trait found in many rhizobacteria. We isolated and screened bacteria from the rhizosphere of wheat for their ACC-degrading ability. The ACC deaminase gene (acdS) isolated from two bacterial isolates through PCR amplification was cloned and sequenced. Nucleotide sequence alignment of these genes with previously reported genes of Pseudomonas sp. strain ACP and Enterobacter cloacae strain UW4 showed variation in their sequences. In the phylogenetic analysis, distinctness of these two genes was observed as a separate cluster. 16S rDNA sequencing of two isolates identified them to be Achromobacter sp. and Pseudomonas stutzeri.
KeywordsPolymerase Chain Reaction Amplification Pseudomonas Fluorescens Pseudomonas Stutzeri Nonspecific Band acdS Gene
The authors are thankful to Department of Science and Technology (DST), India, for the financial grant. The first author thanks Indian Agricultural Research Institute, New Delhi, India, for his PhD fellowship.
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