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Characterization of the Glycine Betaine Biosynthetic Genes in the Moderately Halophilic Bacterium Halobacillus dabanensis D-8T

Current Microbiology volume 57, Article number: 306 (2008) Cite this article

An Erratum to this article was published on 14 October 2009

Abstract

The gene cluster involved in the choline-glycine betaine conversion pathway was cloned from chromosomal DNA of the Gram-positive moderate halophile Halobacillus dabanensis D-8T. Nucleotide sequence analysis revealed four genes, designated gbsT, gbsI, gbsA, and gbsB, which are clustered in a 5.1-kb fragment. After heterologous expression of gbsAB in the Escherichia coli mutant strain PD141, the transformed cells were able to grow in a selective M63 medium containing 0.7 M NaCl and 1 mM choline, in contrast to the mutant strain. Glycine betaine biosynthesis was restored and its accumulation was confirmed by using 13C nuclear magnetic resonance spectroscopy.

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Acknowledgments

We are grateful to Dr. Joaquĭn J. Nieto (University of Seville, Spain) for the kind gift of E. coli strain PD141. This work was supported by a grant from China International Science and Technology Cooperation (2006DFA31060).

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Affiliations

  1. Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing, and Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture, No. 2 Yuanmingyuan West Road, Beijing, 100193, China

    Zhi Jing Gu, Lei Wang, Bo Zhang & Su Sheng Yang

  2. Unité Interactions Plantes-Microorganismes et Santé Végétale, UMR6192 CNRS-INRA-Université de Nice-Sophia Antipolis, Centre INRA Agrobiotech, 06903, Sophia Antipolis, France

    Daniel Le Rudulier

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  1. Zhi Jing Gu
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  2. Lei Wang
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  3. Daniel Le Rudulier
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  4. Bo Zhang
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  5. Su Sheng Yang
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Correspondence to Su Sheng Yang.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s00284-009-9510-z

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Gu, Z.J., Wang, L., Le Rudulier, D. et al. Characterization of the Glycine Betaine Biosynthetic Genes in the Moderately Halophilic Bacterium Halobacillus dabanensis D-8T . Curr Microbiol 57, 306 (2008). https://doi.org/10.1007/s00284-008-9194-9

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  • DOI: https://doi.org/10.1007/s00284-008-9194-9

Keywords

  • Choline
  • Glycine Betaine
  • Halophilic Bacterium
  • Inverse Polymerase Chain Reaction
  • PD141 Cell