Abstract
Lactobacillus fermentum strain L23 produced a small bacteriocin, designated bacteriocin L23, with an estimated molecular mass of < 7000 Da. Isolation, purification, and partial characterization of bacteriocin L23 are described. It displayed a wide inhibitory spectrum including both Gram-negative and Gram-positive pathogenic strains and two species of Candida. The antibacterial activity of cell-free culture supernatant fluid was not affected by catalase or urease but was abolished by the proteolytic enzymes trypsin and protease VI. Bacteriocin L23 was heat stable (60 min at 100°C) and showed inhibitory activity over a wide pH range (4.0 to 7.0). The proteinaceous compound was isolated from cell-free culture supernatant fluid and purified. Crude bacteriocin sample was prepared by a process of ammonium sulfate precipitation, gel filtration, thin-layer chromatography, bioautography, and reversed-phase HPLC.
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Acknowledgments
This work was supported by the Secretaría de Ciencia y Técnica de la Universidad Nacional de Río Cuarto. W.G. is a Career Member of CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas). The authors thank Dr. S. Anderson for editing the manuscript.
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Pascual, L.M., Daniele, M.B., Giordano, W. et al. Purification and Partial Characterization of Novel Bacteriocin L23 Produced by Lactobacillus fermentum L23. Curr Microbiol 56, 397–402 (2008). https://doi.org/10.1007/s00284-007-9094-4
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DOI: https://doi.org/10.1007/s00284-007-9094-4