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Regulation of the Type II Secretion Structural Gene xpsE in Xanthomonas campestris Pathovar campestris by the Global Transcription Regulator Clp

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Abstract

GspE is an important component of the type II secretion system (T2SS) in Gram-negative bacteria that supplies energy for the process of protein secretion. The role of GspE and its interactions with other components have been intensively studied. However, regulation of the gspE gene is poorly understood. In this study, we demonstrated that the transcription of xpsE, a homologue of gspE in Xanthomonas campestris pathovar campestris (Xcc), is upregulated by Clp, a homologue of the cyclic AMP-receptor protein, by directly binding to the xpsE promoter region. Overexpression of the clp gene in Xcc wild-type strain 8004 enhanced the production of XpsE as well as endoglucanase and extracellular polysaccharide (EPS).

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Acknowledgments

This work was supported by the Basic Research Program (The “973” program) of the Ministry of Science and Technology of China (Grant: 2006CB101900) and the National Natural Science Foundation of China (Grant No. 30470036).

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Authors and Affiliations

  1. State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100080, P. R. China

    Chao Ge & Chaozu He

  2. Graduate School of the Chinese Academy of Sciences, Beijing, 100039, P. R. China

    Chao Ge

Authors
  1. Chao Ge
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  2. Chaozu He
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Correspondence to Chaozu He.

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Ge, C., He, C. Regulation of the Type II Secretion Structural Gene xpsE in Xanthomonas campestris Pathovar campestris by the Global Transcription Regulator Clp. Curr Microbiol 56, 122–127 (2008). https://doi.org/10.1007/s00284-007-9081-9

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  • DOI: https://doi.org/10.1007/s00284-007-9081-9

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