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Molecular Diversity of Auricularia polytricha Revealed by Inter-Simple Sequence Repeat and Sequence-Related Amplified Polymorphism Markers

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Abstract

Due to unsatisfying attempts to fingerprint Auricularia polytricha, two different molecular maker systems—Inter-Simple Sequence Repeats (ISSR) and Sequence-related amplified polymorphism (SRAP)—were established and tested to quantify molecular diversity among 19 strains of this fungus. A total of 202 (99.0%) and 459 (95.9%) polymorphic bands were detected by 13 ISSR primers and 14 SRAP primer combinations, respectively. By parsimony method, a phylogenetic tree was constructed based on each analysis; the two trees show that 19 A. polytricha strains were distributed into five or four groups. These results demonstrated that both methods were suitable for discriminating among strains of A. polytricha, and the novel SRAP markers are more efficient and preferable. The result also indicated the high level of genetic diversity of A. polytricha and their relationship between each other. These findings would benefit future research in A. polytricha, especially in breeding and medicine development. It also gives a useful method for fingerprinting of other fungi.

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Acknowledgments

We are grateful to Dinghong Jia for providing A. polytricha strains and invaluable suggestions for this work, and to Wei Wei for expert technical assistance and advice.

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Authors and Affiliations

  1. Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China

    Mengyao Yu, Bo Ma & Zhirong Yang

  2. Cellular and Molecular Lab of Chinese Material Medica, Sichuan Institute of Chinese Material Medica, Chengdu, Sichuan, China

    Mengyao Yu, Xia Luo & Xiaoyan Xu

  3. Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, China

    Linyong Zheng

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  1. Mengyao Yu
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  2. Bo Ma
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  3. Xia Luo
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  4. Linyong Zheng
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  5. Xiaoyan Xu
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  6. Zhirong Yang
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Correspondence to Zhirong Yang.

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Yu, M., Ma, B., Luo, X. et al. Molecular Diversity of Auricularia polytricha Revealed by Inter-Simple Sequence Repeat and Sequence-Related Amplified Polymorphism Markers. Curr Microbiol 56, 240–245 (2008). https://doi.org/10.1007/s00284-007-9067-7

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  • DOI: https://doi.org/10.1007/s00284-007-9067-7

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