Current Microbiology

, Volume 55, Issue 5, pp 409–412 | Cite as

Inhibitory Activity of Probiotic Bacillus subtilis UTM 126 Against Vibrio Species Confers Protection Against Vibriosis in Juvenile Shrimp (Litopenaeus vannamei)

  • José Luis BalcázarEmail author
  • Tyrone Rojas-Luna


The bacterial strain Bacillus subtilis UTM 126 produced antimicrobial activity against pathogenic Vibrio species, including V. alginolyticus, V. parahaemolyticus, and V. harveyi. The probiotic effect of B. subtilis was tested by feeding juvenile shrimp (Litopenaeus vannamei) food supplemented with B. subtilis (105 CFU/g) for 28 days before an immersion challenge with V. harveyi at 105 CFU/mL for 24 h. The treatment with B. subtilis UTM 126 decreased final mortality to 18.25%, compared with 51.75% in the control group. Bacillus subtilis UTM 126 has potential applications for controlling pathogenic V. harveyi in shrimp aquaculture.


Pathogenic control Probiotic Shrimp Vibriosis 



This study was supported by the Ecuadorian government through the IECE.

We thank Dr. Alison Moldenke for helpful and critical discussions.


  1. 1.
    Balcázar JL, de Blas I, Ruiz-Zarzuela I, Cunningham D, Vendrell D, Múzquiz JL (2006) The role of probiotics in aquaculture. Vet Microbiol 114:173–186PubMedCrossRefGoogle Scholar
  2. 2.
    Balcázar JL, Decamp O, Vendrell D, de Blas I, Ruiz-Zarzuela I (2006) Health and nutritional properties of probiotics in fish and shellfish. Microb Ecol Health Dis 18:65–70CrossRefGoogle Scholar
  3. 3.
    Chaurasia B, Pandey A, Palni LMS, Trivedi P, Kumar B, Colvin N (2005) Diffusible and volatile compounds produced by an antagonistic Bacillus subtilis strain cause structural deformations in pathogenic fungi in vitro. Microbiol Res 160:75–81PubMedCrossRefGoogle Scholar
  4. 4.
    Drablos F, Nicholson D, Ronning M (1999) EXAFS study of zinc coordination in Bacitracin A. Biochim Biophys Acta 1431:433–442PubMedGoogle Scholar
  5. 5.
    Gomez-Gil B, Tron-Mayén L, Roque A, Turnbull JF, Inglis V, Guerra-Flores AL (1998) Species of Vibrio isolated from hepatopancreas, haemolymph and digestive tract of a population of healthy juvenile Penaeus vannamei. Aquaculture 163:1–9CrossRefGoogle Scholar
  6. 6.
    Gullian M, Thompson F, Rodríguez J (2004) Selection of probiotic bacteria and study of their immunostimulatory effect in Penaeus vannamei. Aquaculture 233:1–14CrossRefGoogle Scholar
  7. 7.
    Hansen GH, Olafsen JA (1999) Bacterial interactions in early life stages of marine cold water fish. Microb Ecol 38:1–26PubMedCrossRefGoogle Scholar
  8. 8.
    Karunasagar I, Pai R, Malathi GR, Karunasagar I (1994) Mass mortality of Penaeus monodon larvae due to antibiotic-resistant Vibrio harveyi infection. Aquaculture 128:203–209CrossRefGoogle Scholar
  9. 9.
    Moriarty D (1997) The role of microorganisms in aquaculture ponds. Aquaculture 151:333–349CrossRefGoogle Scholar
  10. 10.
    Morikawa M, Ito M, Imanaka T (1992) Isolation of a new surfactin producer Bacillus pumilus A-1, and cloning and nucleotide sequence of the regulator gene, psf-1. J Ferment Bioeng 74:255–261CrossRefGoogle Scholar
  11. 11.
    Perez C, Suarez C, Castro GR (1993) Antimicrobial activity determined in strains of Bacillus circulans cluster. Folia Microbiol 38:25–28CrossRefGoogle Scholar
  12. 12.
    Reid G, Sanders ME, Gaskins HR, Gibson GR, Mercenier A, Rastall RA, Roberfroid MB, Rowland I, Cherbut C, Klaenhammer TR (2003) New scientific paradigms for probiotics and prebiotics. J Clin Gastroenterol 37:105–118PubMedCrossRefGoogle Scholar
  13. 13.
    Rengpipat S, Rukpratanporn S, Piyatiratitivorakul S, Menasaveta P (2000) Immunity enhancement in black tiger shrimp (Penaeus monodon) by a probiont bacterium (Bacillus S11). Aquaculture 191:271–288CrossRefGoogle Scholar
  14. 14.
    Rengpipat S, Phianphak W, Piyatiratitivorakul S, Menasaveta P (1998) Effects of a probiotic bacterium in black tiger shrimp Penaeus monodon survival and growth. Aquaculture 167:301–313CrossRefGoogle Scholar
  15. 15.
    Sugita H, Tanaami H, Kobashi T, Deguchi Y (1981) Bacterial flora of coastal bivalves. Bull Jpn Soc Sci Fish 47:655–661Google Scholar
  16. 16.
    Sugita H, Hirose Y, Matsuo N, Deguchi Y (1998) Production of the antibacterial substance by Bacillus sp. strain NM12, an intestinal bacterium of Japanese coastal fish. Aquaculture 165:269–280CrossRefGoogle Scholar
  17. 17.
    Vaseeharan B, Ramasamy P (2003) Control of pathogenic Vibrio spp. by Bacillus subtilis BT23, a possible probiotic treatment for black tiger shrimp Penaeus monodon. Lett Appl Microbiol 36:83–87PubMedCrossRefGoogle Scholar
  18. 18.
    Verschuere L, Rombaut G, Sorgeloos P, Verstraete W (2000) Probiotic bacteria as biological control agents in aquaculture. Microbiol Mol Biol Rev 64:655–671PubMedCrossRefGoogle Scholar
  19. 19.
    Yilmaz M, Soran H, Beyatli Y (2006) Antimicrobial activities of some Bacillus spp. strains isolated from the soil. Microbiol Res 161:127–131PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  1. 1.Laboratory of Fish Pathology, Faculty of Veterinary SciencesUniversity of ZaragozaZaragozaSpain
  2. 2.Faculty of AquacultureTechnical University of MachalaMachalaEcuador

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