Abstract
Homologous recombination (HR) was found to be so frequent in haloarchaea that its significance in evolution and diversity of this clade of life might have been underestimated. However, so far there has been no report on recombination function carried on plasmid. Here we report that a 4.8-kb SnaBI-PvuII digested segment from pHH205 might carry such a function. Four constructed plasmids: pUN, pUN-205, pUM and pUM-205, with pUN and pUN205 containing NovR gene, pUM and pUM-205 carrying MevR gene, were used to transform Haloferax volcanii DS52 (radA−). The results showed that only pUN-205 and pUM-205 containing the 4.8-kb SnaBI-PvuII digested segment from pHH205 were able to shift NovR and MevR gene into the chromosome of Haloferax volcanii DS52 through HR, whereas those in pUN and pUM could not, which indicated that the segment from pHH205 does contain a recombination function.
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Literature Cited
Baliga NS, Bonneau R, Facciotti MT, Pan M, Glusman G, Deutsch EW, Shannon P, et al. (2004) Genome sequence of Haloarcula marismortui: a halophilic archaeon from the Dead Sea. Genome Res 14:2221–2234
Bolhuis H, Palm P, Wende A, Falb M, Rampp M, Rodriguez-Valera F, Pfeiffer F, Oesterhelt D (2006) The genome of the square archaeon Haloquadratum walsbyi: life at the limits of water activity. BMC Genomics 7:169
Bolt EL, Guy CP (2003) Homologous recombination in Archaea: new Holliday junction helicases. Biochem Soc Trans 31:703–705
Charlebois RL, Lam WL, Cline SW, Doolittle WF (1987) Characterization of pHV2 from Halobacterium volcanii and its use in demonstrating transformation of an archaebacterium. Proc Natl Acad Sci USA 84:8530–8534
Cline SW, Lam WL, Charlebois RL, Schalkwyk LC, Doolittle WF (1989) Transformation methods for halophilic archaeabacteria. Can J Microbiol 35:148–152
Cox MM (2001) Recombinational DNA repair of damaged replication fork in Escherichia coli: questions. Annu Rev Genet 35:53–82
Gerard E, Jolivet E, Prieur D, Forterre P (2001) DNA protection mechanisms are not involved in the radioresistance of the hyperthermophilic archaea Pyrococcus abyssi and P. furiosus. Mol Genet Genomics 266:72–78
Holliday R (1964) A mechanism for gene conversion in fungi. Genet Res Camb 5:282–304
Holmes ML, Dyall-Smith ML (1990) A plasmid vector with a selectable marker for halophilic archaebacteria. J Bacteriol 172:756–761
Holmes ML, Dyall-Smith ML (1991) Mutations in DNA gyrase result in novobiocin resistance in halophilic archaebacteria. J Bacteriol 173:642–648
Holmes ML, Nuttall ST, Dyall-Smith ML (1991) Construction and use of halobacterial shuttle vectors and further studies on haloferax DNA gyrase. J Bacteriol 173:3807–3813
Komori K, Ishino Y (2001) Replication protein A in Pyrococcus furiosus is involved in homologous DNA recombination. J Biol Chem 276:25654–25660
Lam WL, Doolittle WF (1989) Shuttle vectors for the archaebacterium Halobacterium volcanii. Proc Natl Acad Sci USA 86:478–5482
Lam WL, Doolittle WF (1992) Mevinolin-resistant mutations identify a promoter and the gene for a eukaryote-like 3-hydroxy-3-methylglutaryl-coenzyme A reductase in the archaebacterium haloferax volcanii. J Biol Chem 267:5829–5834
Lusetti SL, Cox MM (2002) The bacterial RecA protein and the recombinational DNA repair of stalled replication forks. Annu Rev Biochem 71:71–100
Meselson MS, Radding CM (1975) A general model for genetic recombination. Proc Natl Acad Sci USA 72:358–361
Papke RT, Koenig JE, Rodíguez-Valera F, Doolittle WF (2004) Frequent recombination in a saltern population of Halorubrum. Science 306:1928–1929
Pastink A, Eeken JC, Lohman PH (2001) Genomic integrity and the repair of double-strand DNA breaks. Mutat Res 480–481: 37–50
Rashid N, Morikawa M, Imanaka T (1997) Characterization of a RecA/RAD51 homologue from the hyperthermophilic archaeon Pyrococcus sp. KOD1. Nucleic Acids Res 25:719–726
Reich CI, McNeil LK, Brace JL, Brucker JK, Olsen GJ (2001) Archaeal RecA homologues: different response to DNA-damaging agents in mesophilic and thermophilic Archaea Extremophiles 5:265–275
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
Sandler SJ, Hugenholta P, Schleper C, DeLong EF, Pace NR, Clark AJ (1999) Diversity of radA genes from cultured and uncultured archaea: comparative analysis of putative RadA proteins and their use as a phylogenetic marker. J Bacteriol 181:907–915
Sandler SJ, Satin LH, Samra HS, Clark AJ (1996) recA-like genes from three archaean species with putative protein products similar to Rad51 and Dmc1 proteins of the yeast Saccharomyces cerevisiae. Nucleic Acids Res 24:2125–2132
Seitz EM, Brockman JP, Sandler SJ, Clark AJ, Kowalczykowski SC (1998) RadA protein is an archaeal RecA protein homolog that catalyzes DNA strand exchange. Genes Dev 12:1248–1253
Shen P, Chen YJ (1994) Plasmid from Halobacterium halobium and its restriction map. Chinese J Genet 21:409–416
Shibata T (2001) Functions of homologous DNA recombination. RIKEN Rev 41:21–23
Spies M, Kil Y, Masui R, Kato R, Kujo C, Ohshima T, Kuramitsu S, Lanzov V (2000) The RadA protein from a hyperthermophilic archaeon Pyrobaculum islandicum is a DNA-dependent ATPase that exhibits two disparate catalytic modes, with a transition temperature at 75°C. Eur J Biochem 267:1125–1137
White CI, Haber JE (1990) Intermediates of recombination during mating type switching in Saccharomyces cerevisiae. EMBO J 9:663–673
Woods WG, Dyall-Smith ML (1997) Construction and analysis of recombination-deficient (radA) mutant of Haloferax volcanii. Mol Microbiol 23:791–797
Ye XC, Ou JH, Ni LN, Shi WL, Shen P (2003) Characterization of a novel plasmid from extremely halophilic archaea: nucleotide sequence and function analysis. FEMS Microbiol Lett 221:53–57
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Mei, Y., Chen, D., Sun, D. et al. Identification Homologous Recombination Function from Haloarchaea Plasmid pHH205. Curr Microbiol 55, 76–80 (2007). https://doi.org/10.1007/s00284-007-0043-z
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DOI: https://doi.org/10.1007/s00284-007-0043-z