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Gene Sequence Phylogenies of the Family Microbacteriaceae

Current Microbiology volume 55, pages 42–46 (2007)Cite this article

Abstract

The type strains of 32 species of 13 genera of the family Microbacteriaceae were analysed with respect to gene-coding phylogeny for DNA gyrase subunit B (gyrB), RNA-polymerase subunit B (rpoB), recombinase A (recA), and polyphosphate kinase (ppk). The resulting gene trees were compared with the 16S rRNA gene phylogeny of the same strains. The topology of neighbour-joining and maximum parsimony phylogenetic trees, based on nucleic-acid sequences and protein sequences of housekeeping genes, differed from one another, and no gene tree was identical to that of the 16S rRNA gene tree. Most genera analysed containing >1 strain formed phylogenetically coherent taxa. The three pathovars of Curtobacterium flaccumfaciens clustered together to the exclusion of the type strains of other Curtobacterium species in all DNA - and protein-based analyses. In no tree did the distribution of a major taxonomic marker, i.e., diaminobutyric acid versus lysine and/or ornithine in the peptidoglycan, or acyl type of peptidoglycan, correlate with the phylogenetic position of the organisms. The changing phylogenetic position of Agrococcus jenensis was unexpected: This strain defined individual lineages in the trees based on 16S rRNA and gyrB and showed identity with Microbacterium saperdae in the other three gene trees.

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Affiliations

  1. Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstrasse 7B, 38124, Braunschweig, Germany

    Erko Stackebrandt, Evelyne Brambilla & Kathrin Richert

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  1. Erko Stackebrandt
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  2. Evelyne Brambilla
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  3. Kathrin Richert
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Correspondence to Erko Stackebrandt.

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Stackebrandt, E., Brambilla, E. & Richert, K. Gene Sequence Phylogenies of the Family Microbacteriaceae . Curr Microbiol 55, 42–46 (2007). https://doi.org/10.1007/s00284-006-0569-5

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  • DOI: https://doi.org/10.1007/s00284-006-0569-5

Keywords

  • Peptidoglycan
  • Microbacterium
  • Clavibacter
  • Multilocus Sequence Analysis
  • Leifsonia