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Production and Regulation of Lipase Activity from Penicillium restrictum in Submerged and Solid-State Fermentations

Current Microbiology volume 54, pages 361–365 (2007)Cite this article

Abstract

Different carbon (C) sources, mainly carbohydrates and lipids, have been screened for their capacity to support growth and lipase production by Penicillium restrictum in submerged fermentation (SmF) and in solid-state fermentation (SSF). Completely different physiological behaviors were observed after the addition of easily (oleic acid and glucose) and complex (olive oil and starch) assimilable C sources to the liquid and solid media. Maximal lipolytic activities (12.1 U/mL and 17.4 U/g) by P. restrictum were obtained with olive oil in SmF and in SSF, respectively. Biomass levels in SmF (12.2–14.1 mg/mL) and SSF (7.0–8.0 mg/g) did not varied greatly with the distinct C sources used. High lipase production (12.3 U/g) using glucose was only attained in SSF, perhaps due to the ability of this fermentation process to minimize catabolite repression.

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Acknowledgment

The authors acknowledge the financial support from FAPERJ, CNPq/PROFIX, CNPq/Universal.

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Authors and Affiliations

  1. Biochemistry Department, Institute of Chemistry, Federal University of Rio de Janeiro, Centro de Tecnologia, Bloco A, Lab. 549-2, 21945-900, Rio de Janeiro, RJ, Brazil

    Luciana A. I. de Azeredo & Denise M. G. Freire

  2. Chemical Engineering Program/COPPE, Federal University of Rio de Janeiro, Centro de Tecnologia, sala G-116, 21941-972, Rio de Janeiro, RJ, Brazil

    Patrícia M. Gomes, Geraldo L. Sant’Anna Jr. & Leda R. Castilho

Authors
  1. Luciana A. I. de Azeredo
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  2. Patrícia M. Gomes
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  3. Geraldo L. Sant’Anna Jr.
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  4. Leda R. Castilho
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  5. Denise M. G. Freire
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Correspondence to Denise M. G. Freire.

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de Azeredo, L.A.I., Gomes, P.M., Sant’Anna, G.L. et al. Production and Regulation of Lipase Activity from Penicillium restrictum in Submerged and Solid-State Fermentations. Curr Microbiol 54, 361–365 (2007). https://doi.org/10.1007/s00284-006-0425-7

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  • DOI: https://doi.org/10.1007/s00284-006-0425-7

Keywords

  • Lipase
  • Oleic Acid
  • Lipase Activity
  • Lipolytic Activity
  • Lipase Production