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Effects of Ultraviolet Radiation on the Gram-Positive Marine Bacterium Microbacterium maritypicum

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Abstract

Although extensive information is available on the effect ultraviolet (UV) radiation has on Gram-negative marine bacteria, there is a scarcity of data concerning UV radiation and Gram-positive marine bacteria. The focus of this paper is on Microbacterium maritypicum, with the Gram-negative Vibrio natriegens being used as a standard of comparison. M. maritypicum exhibited growth over a NaCl range of 0–1000 mM, with optimum growth occurring between 0 and 400 mM NaCl. In contrast, V. natriegens grew over a NaCl span of 250–1000 mM, with best growth being observed between 250 and 600 mM NaCl. UV radiation experiments were done using the medium with 250 mM NaCl. For solar (UV-A and B) radiation and log-phase cells, M. maritypicum was determined to be three times more resistant than V. natriegens. For germicidal (UV-C) radiation, the pattern of resistance of the log-phase cells to the lethal effects of the radiation was even more pronounced, with the Gram-positive bacterium being more than 12 to 13 times more resistant. Similar data to the solar and germicidal log-phase UV kill curves were obtained for stationary-phase cells of both organisms. Photoreactivation was observed for both types of cells exposed to UV-C but none for cells treated with UV-A and B. When log phase cells of M.maritypicum were grown at 0.0 and 0.6 M NaCl and exposed to UV-C radiation, no difference in survivorship patterns was noted from that of 0.25 M NaCl grown cells. Although this study has only focused on two marine bacteria, our results indicate that the Gram-positive M. maritypicum could have a built-in advantage for survival in some marine ecosystems.

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Authors and Affiliations

  1. School of Biological Sciences, E-157 Beadle Center, University of Nebraska–Lincoln, Lincoln, NE, 68588-0666, USA

    Patrick D. Williams, Shaundra L. Eichstadt & Eugene L. Martin

  2. Department of Microbiology, Arizona College of Osteopathic Medicine, Glendale, AZ, 85308, USA

    Tyler A. Kokjohn

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  1. Patrick D. Williams
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  2. Shaundra L. Eichstadt
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  3. Tyler A. Kokjohn
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  4. Eugene L. Martin
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Correspondence to Eugene L. Martin.

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Williams, P.D., Eichstadt, S.L., Kokjohn, T.A. et al. Effects of Ultraviolet Radiation on the Gram-Positive Marine Bacterium Microbacterium maritypicum . Curr Microbiol 55, 1–7 (2007). https://doi.org/10.1007/s00284-006-0349-2

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  • DOI: https://doi.org/10.1007/s00284-006-0349-2

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