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Correlation Between Antifungal Agent Phenazine-1-Carboxylic Acid and Pyoluteorin Biosynthesis in Pseudomonas sp. M18

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Abstract

Biosynthesis and secretion of two different types of antifungal compound [phenazine-1-carboxylic acid (PCA) and pyoluteorin (Plt) in Pseudomonas sp. M18] contribute to its suppression of soil-borne root pathogens. To better understand the correlation between two antifungal agents in secondary metabolism, a DNA fragment covering partial pltC and pltD coding sequences was obtained by screening the genomic library of Pseudomonas sp. M18. A mutant, M18T, was then constructed by insertion of the aacC1 gene cassette (encoding gentamycin resistance). With the same methods, one PCA biosynthetic gene cluster was insertionally inactivated and a mutant M18Z1 was created. The mutant strain M18T produces no Plt and the same amount of PCA in comparison with the wild-type strain M18. The mutant M18Z1, however, produces less PCA but more Plt than the wild-type strain M18. According to the documented data on strain M18, it is suggested that production of PCA is not influenced by Plt yield, but Plt biosynthesis is influenced by an alteration of PCA production.

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Acknowledgments

We thank Dr. Stephan Heeb for kindly providing the plasmids used in this study. This work was financially supported by grant No.30370041 from the National Science Foundation.

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Authors and Affiliations

  1. College of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai, 200240, People’s Republic of China

    Yi-He Ge

  2. Department of Biological Science, Shangqiu Normal College, Shangqiu, 476000, Henan province, People’s Republic of China

    Dong-Li Pei

  3. College of Life Science, Ludong University, Yantai, 264000, Shandong province, People’s Republic of China

    Yan-Hong Zhao, Wei-Wei Li & Shu-Fang Wang

  4. College of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai, 200240, People’s Republic of China

    Yu-Quan Xu

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  1. Yi-He Ge
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Correspondence to Yu-Quan Xu.

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Ge, YH., Pei, DL., Zhao, YH. et al. Correlation Between Antifungal Agent Phenazine-1-Carboxylic Acid and Pyoluteorin Biosynthesis in Pseudomonas sp. M18. Curr Microbiol 54, 277–281 (2007). https://doi.org/10.1007/s00284-006-0317-x

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  • DOI: https://doi.org/10.1007/s00284-006-0317-x

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