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Adaptational Changes in Lipids of Bradyrhizobium SEMIA 6144 Nodulating Peanut as a Response to Growth Temperature and Salinity

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Abstract

Phospholipids provide the membrane with its barrier function and play a role in a variety of processes in the bacterial cell, as responding to environmental changes. The aim of the present study was to characterize the physiological and metabolic response of Bradyrhizobium SEMIA 6144 to saline and temperature stress. This study provides metabolic and compositional evidence that nodulating peanut Bradyrhizobium SEMIA 6144 is able to synthesize fatty acids, to incorporate them into its phospholipids (PL), and then modify them in response to stress conditions such as temperature and salinity. The fatty acids were formed from [1-14C]acetate and mostly incorporated in PL (95%). Phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and cardiolipin (CL) were found to be the major phospholipids in the bacteria analyzed. The amount and the labeling of each individual PL was increased by NaCl, while they were decreased by temperature stress. The amount of PC, PE, and PG under the combined stresses decreased, as in the temperature effect. The results indicate that synthesized PL of Bradyrhizobium SEMIA 6144 are modified under the tested conditions. Because in all conditions tested the PC amount was always modified and PC was the major PL, we suggest that this PL may be involved in the bacteria response to environmental conditions.

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Acknowledgments

We thank Dr. Carlos Domenech for advice and discussions and the technical assistance of Microbiologist Mariela Woelke. We also thank the contribution of language consultant Professor Iliana A. Martínez. This work was supported by SECyT, UNRC, Río Cuarto, Córdoba, Argentina and ANPCyT, Argentina. Daniela Medeot and Marta Dardanelli are fellows of CONICET Argentina.

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Authors and Affiliations

  1. Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, CPX5804BYA, Río Cuarto, Córdoba, Argentina

    Daniela B. Medeot, Miguel A. Bueno, Marta S. Dardanelli & Mirta García de Lema

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  1. Daniela B. Medeot
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  2. Miguel A. Bueno
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  3. Marta S. Dardanelli
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  4. Mirta García de Lema
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Correspondence to Mirta García de Lema.

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Medeot, D.B., Bueno, M.A., Dardanelli, M.S. et al. Adaptational Changes in Lipids of Bradyrhizobium SEMIA 6144 Nodulating Peanut as a Response to Growth Temperature and Salinity. Curr Microbiol 54, 31–35 (2007). https://doi.org/10.1007/s00284-006-0233-0

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  • DOI: https://doi.org/10.1007/s00284-006-0233-0

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