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Rapid Screening of Plasmid-Encoded Small hsp-Genes in Streptococcus thermophilus

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Abstract

A new rapid procedure for detection of small heat shock protein genes (shsp) was developed. Using PCR-based molecular approach, single colonies of 49 Streptococcus thermophilus industrial and artisanal starters were examined. Five strains contained plasmid-encoded shsp. The nucleotide sequence analysis revealed that the genes are very conservative, as only a few nucleotide substitutions were noticed. It was shown that all new isolated plasmids belong to the pC194 family of rolling circle replicating (RCR) plasmids. We concluded that shsp genes are always inherited together with pC194-type replicative region. The viability of plasmid-bearing and plasmid-free derivatives of S. thermophilus strain ST2980 under heat shock condition was studied.

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Acknowledgments

This study was financed by the National Council for Scientific Research, Republic of Bulgaria, Grant K 1307/2003.

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  1. Department of Microbial Genetics, Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. bl. 26, 1113, Sofia, Bulgaria

    Penka M. Petrova & Dilnora E. Gouliamova

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  1. Penka M. Petrova
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  2. Dilnora E. Gouliamova
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Correspondence to Penka M. Petrova or Dilnora E. Gouliamova.

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Petrova, P.M., Gouliamova, D.E. Rapid Screening of Plasmid-Encoded Small hsp-Genes in Streptococcus thermophilus . Curr Microbiol 53, 422–427 (2006). https://doi.org/10.1007/s00284-006-0175-6

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  • DOI: https://doi.org/10.1007/s00284-006-0175-6

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