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Production of Cellulase-Free Xylanase from Bacillus megaterium by Solid State Fermentation for Biobleaching of Pulp

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Abstract

Xylanase production from B. megaterium was enhanced using solid state fermentation with respect to the use of solid substrate, moistening solution, moisture content, inoculum, sugars, soyabean meal, amino acids, and extraction with surfactant. An increase of ≈423-fold in xylanase production and complete suppression of CMCase production was achieved over submerged liquid fermentation. Biobleaching using this cellulase-free xylanase, 8 U/g of oven dried pulp of 10% consistency, showed 8.12% and 1.16% increase in brightness and viscosity, 13.67% decrease in kappa number, and 31% decrease in chlorine consumption at the CD stage.

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Authors and Affiliations

  1. Department of Microbiology, Punjab University, Chandigarh, 160014, India

    Indu Sindhu, Sanjay Chhibber & Prince Sharma

  2. Department of Biotechnology, Punjab University, Chandigarh, 160014, India

    Neena Capalash

Authors
  1. Indu Sindhu
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  2. Sanjay Chhibber
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  3. Neena Capalash
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  4. Prince Sharma
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Correspondence to Prince Sharma.

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Sindhu, I., Chhibber, S., Capalash, N. et al. Production of Cellulase-Free Xylanase from Bacillus megaterium by Solid State Fermentation for Biobleaching of Pulp. Curr Microbiol 53, 167–172 (2006). https://doi.org/10.1007/s00284-006-0051-4

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  • DOI: https://doi.org/10.1007/s00284-006-0051-4

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