Abstract
The Escherichia coli NADP+-dependent isocitrate dehydrogenase (IDH; EC 1.1.1.42), encoded by an icd gene, is a tricarboxylic acid (TCA) cycle enzyme responsible for the oxidative decarboxylation of isocitrate to α-ketoglutarate. In order to examine how the icd gene expression is regulated, an icd-lacZ reporter fusion was constructed. While the icd gene was induced in exponential growth phase, it was repressed in stationary growth phase. Genetic inactivation of an rpoS gene, whose product is an alternative sigma factor, induced the icd gene expression approximately 4.8 times more in the stationary phase and the IDH enzyme activity in the rpoS mutant was 3.2 times higher than that in the wild type, indicating that the RpoS factor acts as a negative regulator of the icd gene expression in the stationary phase.
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This work was supported by the Nuclear Research and Development Program from the Ministry of Science and Technology (MOST) of Republic of Korea.
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Jung, I.L., Kim, S.K. & Kim, I.G. The RpoS-Mediated Regulation of Isocitrate Dehydrogenase Gene Expression in Escherichia coli. Curr Microbiol 52, 21–26 (2006). https://doi.org/10.1007/s00284-005-8006-8
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DOI: https://doi.org/10.1007/s00284-005-8006-8