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Purification, Characterization, and Antifungal Activity of Chitinase from Streptomyces venezuelae P10

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Abstract

Streptomyces venezuelae P10 could produce extracellular chitinase in a medium containing 0.6% colloidal chitin that was fermented for 96 hours at 30°C. The enzyme was purified to apparent homogeneity with 80% saturation of ammonium sulfate as shown by chitin affinity chromatography and DEAE-cellulose anion-exchange chromatography. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) of the enzyme showed a molecular weight of 66 kDa. The chitinase was characterized, and antifungal activity was observed against phytopathogens. Also, the first 15 N-terminal amino-acid residues of the chitinase were determined. The chitin hydrolysed products were N-acetylglucosamine and N, N’-diacetylchitobiose.

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Acknowledgments

We gratefully acknowledge the financial support received from DBT, Ministry of S & T, and UGC, Government of India. We are also thankful to Dr. A. K. Ghosh, IICB, Kolkata, India, for technical support.

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  1. Division of Microbiology, School of Life Sciences, Visva-Bharati (Central University), Santiniketan, 731235, India

    G. Mukherjee & S. K. Sen

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  1. G. Mukherjee
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  2. S. K. Sen
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Correspondence to S. K. Sen.

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Mukherjee, G., Sen, S. Purification, Characterization, and Antifungal Activity of Chitinase from Streptomyces venezuelae P10 . Curr Microbiol 53, 265–269 (2006). https://doi.org/10.1007/s00284-005-0412-4

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  • DOI: https://doi.org/10.1007/s00284-005-0412-4

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