Current Microbiology

, Volume 53, Issue 6, pp 462–466 | Cite as

Haemagglutination Induced by Bordetella pertussis Filamentous Haemagglutinin Adhesin (FHA) Is Inhibited by Antibodies Produced Against FHA430–873 Fragment Expressed in Lactobacillus casei

  • Débora Colombi
  • Maria L. S. Oliveira
  • Ivana B. Campos
  • Vicente Monedero
  • Gaspar Pérez-Martinez
  • Paulo L. HoEmail author


Filamentous haemagglutinin adhesin (FHA) is an important virulence factor from Bordetella pertussis related to the adhesion and spread of the bacteria through the respiratory tract. Three distinct domains have been characterized in mature FHA, and among them, the FHA442–863 fragment was suggested to be responsible for the heparin-binding activity. In this study, we cloned the gene encoding the HEP fragment (FHA430–873) in a Lactobacillus casei–inducible expression vector based on the lactose operon. The recombinant bacteria, transformed with the resulting construct (L. casei-HEP), were able to express the heterologous protein depending on the sugar added to the culture. Subcutaneous inoculation of L. casei-HEP in Balb/C mice, using the cholera toxin B subunit as adjuvant, induced systemic anti-HEP antibodies that were able to inhibit in vitro erythrocyte haemagglutination induced by FHA. This is the first example of a B. pertussis antigen produced in lactic acid bacteria and opens new perspectives for alternative vaccine strategies against whooping cough.


Pertussis Pertussis Vaccine Bordetella Pertussis Pool Seron Acellular Vaccine 
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This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Fundação Butantan and CNPq .

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Débora Colombi
    • 1
    • 2
  • Maria L. S. Oliveira
    • 1
  • Ivana B. Campos
    • 1
    • 3
  • Vicente Monedero
    • 4
  • Gaspar Pérez-Martinez
    • 4
  • Paulo L. Ho
    • 1
    • 2
    • 3
    • 5
    Email author
  1. 1.Centro de BiotecnologiaInstituto ButantanSão PauloBrazil
  2. 2.Instituto de Quimica, USPSão PauloBrazil
  3. 3.Interunidades em BiotecnologiaInstituto de Ciências Biomédicas, Universidade de São PauloSão PauloBrazil
  4. 4.Instituto de Agroquímica y Tecnología de Alimentos (CSIC)ValenciaSpain
  5. 5.Instituto de BiociênciasUNESPBotucatuBrazil

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