Advertisement

Current Microbiology

, Volume 53, Issue 6, pp 462–466 | Cite as

Haemagglutination Induced by Bordetella pertussis Filamentous Haemagglutinin Adhesin (FHA) Is Inhibited by Antibodies Produced Against FHA430–873 Fragment Expressed in Lactobacillus casei

  • Débora Colombi
  • Maria L. S. Oliveira
  • Ivana B. Campos
  • Vicente Monedero
  • Gaspar Pérez-Martinez
  • Paulo L. HoEmail author
Article

Abstract

Filamentous haemagglutinin adhesin (FHA) is an important virulence factor from Bordetella pertussis related to the adhesion and spread of the bacteria through the respiratory tract. Three distinct domains have been characterized in mature FHA, and among them, the FHA442–863 fragment was suggested to be responsible for the heparin-binding activity. In this study, we cloned the gene encoding the HEP fragment (FHA430–873) in a Lactobacillus casei–inducible expression vector based on the lactose operon. The recombinant bacteria, transformed with the resulting construct (L. casei-HEP), were able to express the heterologous protein depending on the sugar added to the culture. Subcutaneous inoculation of L. casei-HEP in Balb/C mice, using the cholera toxin B subunit as adjuvant, induced systemic anti-HEP antibodies that were able to inhibit in vitro erythrocyte haemagglutination induced by FHA. This is the first example of a B. pertussis antigen produced in lactic acid bacteria and opens new perspectives for alternative vaccine strategies against whooping cough.

Keywords

Pertussis Pertussis Vaccine Bordetella Pertussis Pool Seron Acellular Vaccine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Fundação Butantan and CNPq .

Literature Cited

  1. 1.
    Alonso S, Reveneau N, Pethe K, Locht C (2002) Eighty-kilodalton N-terminal moiety of Bordetella pertussis filamentous hemagglutinin: Adherence, immnogenicity and protective role. Infect Immun 70:4142–4147PubMedCrossRefGoogle Scholar
  2. 2.
    Arêas APM, Oliveira MLS, Miyaji EN, Leite LCC, Dias WO, Aires K, et al. (2004) Expression and characterization of cholera toxin B-pneumococcal surface adhesin A fusion protein in Escherichia coli: Ability of CTB-PsaA to induce humoral immune response in mice. Biochem Bioph Res Comm 321:192–196CrossRefGoogle Scholar
  3. 3.
    Colombi D, Horton DSPQ, Oliveira MLS, Sakauchi MA, Ho PL (2004) Antibodies produced against a fragment of filamentous hemagglutination (FHA) of Bordetella pertussis are able to inhibit hemagglutination induced by the whole adhesion. FEMS Microbiol Lett 240:41–47PubMedCrossRefGoogle Scholar
  4. 4.
    Gosalbes MJ, Monedero V, Perez-Martinez G (1999) Elements involved in catabolite repression and substrate induction of the lactose operon in Lactobacillus casei. J Bacteriol 181:3928–3934PubMedGoogle Scholar
  5. 5.
    Gosalbes MJ, Pérez-Arellano I, Esteban CD, Galán JL, Pérez-Martínez G (2001) Use of lac elements for gene expression in Lactobacillus casei. Lait 81:29–35CrossRefGoogle Scholar
  6. 6.
    Grangette C, Müller-Alouf H, Goudercourt D, Geoffroy MC, Turneer M, Mercenier A (2001) Mucosal immune response and protection against tetanus toxin after intranasal immunization with recombinant Lactobacillus plantarum. Infect Immun 69:1547–1553PubMedCrossRefGoogle Scholar
  7. 7.
    Hanniffy S, Wiedermann U, Repa A, Mercenier A, Daniel C, Fioramonti J, et al. (2004) Potential and opportunities for use of recombinant lactic acid bacteria in human health. Adv Appl Microbiol 56:1–64PubMedCrossRefGoogle Scholar
  8. 8.
    Olin P, Rasmussen F, Gustafsson L, Hallander HO, Heijbel H (1997) Randomised controlled trial of two-component, three-component, and five-component acellular pertussis vaccines compared with whole-cell pertussis vaccine. Ad Hoc Group for the Study of Pertussis Vaccines. Lancet 29:1569–1577CrossRefGoogle Scholar
  9. 9.
    Oliveira MLS, Arêas APM, Campos IB, Monedero V, Perez-Martínez G, Miyaji EN, et al. (2006) Induction of systemic and mucosal immune response and decrease of Streptococcus pneumoniae colonization by nasal inoculation of mice with recombinant lactic acid bacteria expressing the Pneumococcal Surface Antigen A (PsaA). Microbes Infect 8:1016–1024PubMedCrossRefGoogle Scholar
  10. 10.
    Posno M, Leer RJ, van Luijk N, van Giezen MJF, Heuvelmans PTHM, Lokman BC, et al. (1991) Incompatibility of lactobacillus vectors with replicons derived from small cryptic lactobacillus plasmids and segregational instability of the introduced vectors. Appl Environ Microbiol 57:1822–1828PubMedGoogle Scholar
  11. 11.
    van der Berg BM, Beekhuisen H, Willems RJL, Mooi FR, Furth R (1999) Role of Bordetella pertussis virulence factors in adherence to epithelial cell lines derived from the human respiratory tract. Infect Immun 67:1056–1062PubMedGoogle Scholar
  12. 12.
    Watanabe M, Komatsu E, Abe K, Iyama S, Sato T, Nagai M (2002) Efficacy of pertussis components in an acellular vaccine, as assessed in a murine model of respiratory infection and a murine intracerebral challenge model. Vaccine 20:1429–1434PubMedCrossRefGoogle Scholar
  13. 13.
    Wilson DR, Siebers A, Finlay BB (1998) Antigenic analysis of Bordetella pertussis filamentous hemagglutinin polyclonal antibodies. Infect Immun 66:4884–4894PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Débora Colombi
    • 1
    • 2
  • Maria L. S. Oliveira
    • 1
  • Ivana B. Campos
    • 1
    • 3
  • Vicente Monedero
    • 4
  • Gaspar Pérez-Martinez
    • 4
  • Paulo L. Ho
    • 1
    • 2
    • 3
    • 5
    Email author
  1. 1.Centro de BiotecnologiaInstituto ButantanSão PauloBrazil
  2. 2.Instituto de Quimica, USPSão PauloBrazil
  3. 3.Interunidades em BiotecnologiaInstituto de Ciências Biomédicas, Universidade de São PauloSão PauloBrazil
  4. 4.Instituto de Agroquímica y Tecnología de Alimentos (CSIC)ValenciaSpain
  5. 5.Instituto de BiociênciasUNESPBotucatuBrazil

Personalised recommendations