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Screening of Compactin-Resistant Microorganisms Capable of Converting Compactin to Pravastatin

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Abstract

A simple method of using compactin for effective screening of microbial strains with high hydroxylation activity at the 6β position of compactin was developed. Agar plates containing different carbon sources and 500 μg compactin mL−1 were used to screen the microorganisms that can convert compactin to pravastatin. About 100 compactin-resistant strains were isolated from the Basal agar containing 7% (w/v) mannitol as a carbon source, in which two bacteria, Pseudomocardia autotrophica BCRC 12444 and Streptomyces griseolus BCRC 13677, capable of converting compactin to pravastatin with the yield of 20 and 32% (w/w), respectively, were found. High-performance liquid chromatography using C-18 column and two sequential mobile phases, 30% and 50% (v/v) acetonitrile, was also established to simultaneously determine the concentration of compactin and pravastatin in the culture broth. As such, about 2% of target microorganisms could be obtained from the screening program.

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Acknowledgments

This work was supported by grants NSC 92-2311-B-005-016 from the National Science Council of the Republic of China and CMU93-MT-06 from the China Medical University.

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Authors and Affiliations

  1. Departments of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, 404, Taiwan

    Chao-Hsien Chen

  2. Department of Food and Nutrition, HungKuang University, Taichung, 433, Taiwan

    Hui-Yu Hu

  3. Institute of Molecular Biology, National Chung Hsing University, Taichung, 402, Taiwan

    Chao-Hsien Chen, Yen-Ching Cho & Wen-Hwei Hsu

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  1. Chao-Hsien Chen
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  2. Hui-Yu Hu
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  4. Wen-Hwei Hsu
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Correspondence to Hui-Yu Hu.

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Chen, CH., Hu, HY., Cho, YC. et al. Screening of Compactin-Resistant Microorganisms Capable of Converting Compactin to Pravastatin. Curr Microbiol 53, 108–112 (2006). https://doi.org/10.1007/s00284-005-0276-7

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  • DOI: https://doi.org/10.1007/s00284-005-0276-7

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