Current Microbiology

, Volume 52, Issue 3, pp 197–203 | Cite as

Identification of AHBA Biosynthetic Genes Related to Geldanamycin Biosynthesis in Streptomyces hygroscopicus 17997

  • Weiqing He
  • Linzhuan Wu
  • Qunjie Gao
  • Yu Du
  • Yiguang WangEmail author


To clone and study the geldanamycin biosynthetic gene cluster in Streptomyces hygroscopicus 17997, we designed degenerate primers based on the conserved sequence of the ansamycin 3-amino-5-hydroxybenzoic acid (AHBA) synthase gene. A 755-bp polymerase chain reaction product was obtained from S. hygroscopicus 17997 genomic DNA, which showed high similarity to ansamycin AHBA synthase genes. Through screening the cosmid library of S. hygroscopicus 17997, two loci of separated AHBA biosynthetic gene clusters were discovered. Comparisons of sequence homology and gene organization indicated that the two AHBA biosynthetic gene clusters could be divided into a benzenic and a naphthalenic subgroup. Gene disruption demonstrated that the benzenic AHBA gene cluster is involved in the biosynthesis of geldanamycin. However, the naphthalenic AHBA genes in the genome of Streptomyces hygroscopicus 17997 could not complement the deficiency of the benzenic AHBA genes. This is the first report on the AHBA biosynthetic gene cluster in a geldanamycin-producing strain.


Biosynthetic Gene Biosynthetic Gene Cluster Rifamycin Geldanamycin BamHI Fragment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by a grant from the National Department of Sciences and Technology under Preliminary Basic Research 973 project in 2001 (N0.2001CCA00500).

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Weiqing He
    • 1
  • Linzhuan Wu
    • 1
  • Qunjie Gao
    • 1
  • Yu Du
    • 1
  • Yiguang Wang
    • 1
    Email author
  1. 1.Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences, Peking Union Medical CollegeChina

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