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Non-Invasive Serological Detection of Helicobacter pylori Antigens in Adult Hong Kong Chinese Non-Ulcer Dyspepsia Patients

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Abstract

Objective

The aim of our study was to evaluate the accuracy of the serology test kit (Signify H. pylori test), an office-based serological test and to compare relative performances with endoscopy, including biopsy urease tests (Clotest), histology, and culture when applied to dyspeptic adult patients in Hong Kong Chinese.

Methods

Prospective clinical trial was conducted at the Alice Ho Miu ling Nethersole Hospital (AHNH) outpatient endoscopy center from June to December 2004 and included adult Chinese patients who attended the endoscopy center. A research nurse interviewed consenting patients and whole blood samples were obtained via simple fingerstick were then and transferred to Signify test kits. The results were read after 5 minutes. The presence of two distinct lines within the result-reading window was treated as a positive result; a single distinct line was treated as a negative result. All patients underwent upper gastroduodenal endoscopy, and antral/antrum gastric biopsy specimens were taken and then processed for microbiological cultures.

Results

A total of 384 patients were recruited in the study and 131 (34.7%) patients were tested positive for H. pylori. Among them, 62 patients (47.3%) were detected by the Signify H. pylori test. The Signify serology test kit was 52.7% sensitive, 87.9% specific, and 75.7% accurate, respectively in the current study.

Conclusion

The Signify H. pylori test showed a sub-optimal correlation between the presence of H. pylori infection and the serological test results in a Hong Kong Chinese population.

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Literature Cited

  1. International Agency for Cancer Research (1994) Shistosomes, liver flukes and Helicobacter pylori. IARC Monographs on the Evaluation of Cancinogenic Risks to Humans. Lyon, France IARC, p 61

    Google Scholar 

  2. Eck M, Schmausser B, Hass R, Greiner A, Czub S, Muller-Hermelink H (1997) MALT-type lymphoma of the stomach is associated with Helicobacter pylori strains expressing the CagA protein. Gastroenterology 112:1482–1486

    Article  PubMed  CAS  Google Scholar 

  3. Forman D (1998) Helicobacter pylori: the gastric cancer problem. Gut 43:533–534

    Article  Google Scholar 

  4. Suerbaum S, Michetti P (2002) Helicobacter pylori infection. N Engl J Med 347:1175–1186

    Article  PubMed  CAS  Google Scholar 

  5. Graham DY, Qureshi WA (2001) Markers of infection, In Mobley HLT, Mendz GL, Hazell SL (eds) Helicobacter pylori: physiology and genetics. ASM Press, Washington, DC: pp 499–510

    Google Scholar 

  6. Westblom TU, Bhatt BD (1999) Diagnosis of Helicobacter pylori infection. Eur J Gastroenterol Hepatol 8:501–507

    Google Scholar 

  7. Kosunen TU, Seppala K, Sarna S, Siponen P (1992) Diagnostic value of decreasing IgG, IgA, and IgM antibody titres after eradication of Helicobacter pylori. Lancet Apr 11;339(8798):893–895

    Google Scholar 

  8. Laine LA, Lewin D, Naritoko W, et al. (1996) Prospective comparison of commercially available rapid urease tests for the diagnosis of Helicobacter pylori. Gastrointest Endosc 44:429–432.

    Article  PubMed  CAS  Google Scholar 

  9. Wong WM, Lam SK, Xia HH, et al. (2002) New methods for diagnosis of helicobacter pylori infection and determination of fecal consistency: Accuracy of a new near patient test for the diagnosis of Helicobacter pylori infection in Chinese. J Gastroenterol Hepatol 17:1272–1277.

    Article  PubMed  Google Scholar 

  10. Dhar R, Mustafa AS, Dhar PM, et al. (1998) Evaluation and comparison of two immunodiagnostic assays for Helicobacter pylori antibodies with culture results. Diagn Microbiol Infect Dis 30(1):1–6.

    Article  PubMed  CAS  Google Scholar 

  11. Hook-Nikanne J, Perez-Perez GI, Blaser MJ (1997) Antigenic characterization of Helicobacter pylori strains from different parts of the world. Clin Diagn Lab Immunol 4:592–597

    PubMed  CAS  Google Scholar 

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Acknowledgments

This study was supported by the Direct Grant for Research, Faculty of Medicine, The Chinese University of Hong Kong.

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Authors and Affiliations

  1. School of Pharmacy, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

    Vivian W.Y. Lee, Andrew K.W. Chan & Kenneth K.C. Lee

  2. Department of Medicine and Therapeutics, Division of Gastroenterology and Hepatology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

    Francis K.L. Chan

Authors
  1. Vivian W.Y. Lee
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  2. Andrew K.W. Chan
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  3. Kenneth K.C. Lee
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  4. Francis K.L. Chan
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Corresponding author

Correspondence to Vivian W.Y. Lee.

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Lee, V.W., Chan, A.K., Lee, K.K. et al. Non-Invasive Serological Detection of Helicobacter pylori Antigens in Adult Hong Kong Chinese Non-Ulcer Dyspepsia Patients. Curr Microbiol 54, 249–253 (2007). https://doi.org/10.1007/s00284-005-0179-7

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  • DOI: https://doi.org/10.1007/s00284-005-0179-7

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