Abstract
Streptomyces sp.139 produces an exopolysaccharide (EPS) designated Ebosin with remarkable anti-rheumatic arthritis activity in vivo. The ste (Streptomyces eps) gene cluster required for Ebosin biosynthesis has been identified. According to similarities with other proteins in the database, ste22 shows high homology with glycosyltransferases originated from different microorganisms. In this study, the ste22 gene was disrupted by double crossover via homologous recombination. The EPS produced by the mutant strain Streptomyces sp.139 (ste22−) has a different monosaccharide composition profile in comparison with that of Ebosin. This derivative of Ebosin retained the original antagonistic activity of IL-1R in vitro but lost the bioactivities of anti-inflammation and pain relief in vivo.
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This research has been supported by a grant from the Natural Science Foundation of China (NSFC 30370038) and by a grant from the Chinese National 863 High Technology Project (Grant number 2001AA214091).
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Zhang, T., Wang, L., Xu, G. et al. Disruption of the ste22 Gene Encoding a Glycosyltransferase and Its Function in Biosynthesis of Ebosin in Streptomyces sp. 139. Curr Microbiol 52, 55–59 (2006). https://doi.org/10.1007/s00284-005-0096-9
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DOI: https://doi.org/10.1007/s00284-005-0096-9