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The Major Aeromonas veronii Outer Membrane Protein: Gene Cloning and Sequence Analysis

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Abstract

The gene encoding the major outer membrane protein (OMP) from Aeromonas veronii, Omp38, was cloned and characterized. Sequence analysis revealed an open reading frame of 1,047 nucleotides coding for a primary protein of 349 amino acids with a 20–amino-acid signal peptide at the N-terminal and the consensus sequence Ala-X-Ala (Ala-Asn-Ala) as the signal peptidase I recognition site. The mature protein is composed of 329 amino acids with a calculated molecular mass of 36,327 Da. The degree of identity of the deduced Omp38 amino acid sequence to porins from enteric bacteria (OmpF, PhoE, and OmpC) was only 30%. Nevertheless, Omp38 possesses typical features of Gram-negative porins, including acidic pI, high glycine and low proline content, no cysteine residues, and a carboxy-terminal Phe. On the basis of PhoE-OmpF three-dimensional structure and the Kyte-Doolittle hydrophobicity analysis, it seems likely that Omp38 secondary structure consists of 16 antiparallel β-strands and 8 loops. Phylogenetic analyses among Omp38 and related porins from Gram-negative bacteria originate well-defined clusters that agree with the taxonomy of the corresponding organisms.

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Acknowledgments

This work was supported by Centro de Investigaciones Biológicas del Noroeste (CIBNOR) and Consejo Nacional de Ciencia y Techología (CONACyT) research grants. R.C.V.J. is grateful to the University of Rhade Island (URI) Foundation for additional funds for travel. We also thank to Dr. Possani and Dr. Egly for peptide sequencing.

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Authors and Affiliations

  1. Departamento de Patología Marina, Centro de Investigaciones Biológicas del Noroeste, Av. Mar Bermejo 195, Col. Playa Palo Santa Rita, P. O. Box 128, La Paz, BCS, México

    Roberto Carlos Vázquez-Juárez, Norma Hernández & Felipe Ascencio

  2. Department of Fisheries, Animal and Veterinary Science, University of Rhode Island, 23 Woodward Hall, Kingston, RI, 0288, USA

    Marta Gómez-Chiarri

  3. Departamento de Bioquímica, Facualtad de Medicina, Universidad Autónoma de Nuevo León, Av. Madero y Aguirre Pequeño, Col. Mitras Centro, Monterrey, NL, 64460, México

    Hugo Barrera-Saldaña

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  1. Roberto Carlos Vázquez-Juárez
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  2. Marta Gómez-Chiarri
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  3. Hugo Barrera-Saldaña
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  4. Norma Hernández
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  5. Felipe Ascencio
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Correspondence to Roberto Carlos Vázquez-Juárez.

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Vázquez-Juárez, R.C., Gómez-Chiarri, M., Barrera-Saldaña, H. et al. The Major Aeromonas veronii Outer Membrane Protein: Gene Cloning and Sequence Analysis. Curr Microbiol 51, 372–378 (2005). https://doi.org/10.1007/s00284-005-0054-6

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  • DOI: https://doi.org/10.1007/s00284-005-0054-6

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