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Genetic Variation in Clones of Pseudomonas pseudoalcaligenes After Ten Months of Selection in Different Thermal Environments in the Laboratory

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Abstract

The random amplification of polymorphic DNA (RAPD) method was used to examine genetic variation in experimental clones of Pseudomonas pseudoalcaligenes in two experimental groups, as well as their common ancestor. Six clones derived from a single colony of P. pseudoalcaligenes were cultured in two different thermal regimes for 10 months. Three clones in the Control group were cultured at constant temperature of 35°C and another three clones in the High Temperature (HT) group were propagated at incremental temperature ranging from 41 to 47°C for 10 months. A total of 45 RAPD primers generated 146 polymorphic markers. Analysis of molecular variance (AMOVA) revealed mild (11%) but significant (P < 0.001) genetic difference between the Control and the HT clones. Phylogenetic analysis based on pairwise genetic distances showed that the HT clones were more divergent from the ancestor and from each other than the Control clones, implying that the HT clones of P. pseudoalcaligenes may have evolved faster than the Control clones.

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Acknowledgments

This study was supported by RGC grants from the Hong Kong Research Grant Council (HKU7265/00M, HKU7212/01M) to X.X. We thank S.G. Wu for providing the original strain, and R.B. Huey and A. Danchin for comments and discussion.

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Authors and Affiliations

  1. Department of Ecology & Biodiversity, University of Hong Kong, Hong Kong, China

    Bihong Shi

  2. Department of Biology, University of Ottawa, 150 Louis Pasteur, P.O. Box 450, Station A, Ottawa, Ontario, Canada, K1N 6N5

    Xuhua Xia

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  1. Bihong Shi
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  2. Xuhua Xia
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Correspondence to Bihong Shi.

Appendix

Appendix

Fig. A1
figure 2

Banding patterns of RAPD amplifications of P. pseudoalcaligenes clones. M, λ DNA EcoRI/HindIII marker; C, the negative control; lanes 1 to 7, the clones A, C1, C2, C3, HT1, HT2, and HT3, respectively. The upper rows are primers used corresponding to the bands amplified.

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Shi, B., Xia, X. Genetic Variation in Clones of Pseudomonas pseudoalcaligenes After Ten Months of Selection in Different Thermal Environments in the Laboratory. Curr Microbiol 50, 238–245 (2005). https://doi.org/10.1007/s00284-004-4449-6

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