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Myxobacterial Biodiversity in an Established Oak-Hickory Forest and a Savanna Restoration Site

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Abstract

The purpose of this study was to establish the myxobacterial biodiversity of an established oak-hickory forest and a savanna restoration site that has been cut and subsequently burned on four occasions between 1993 and 1998 in an attempt to restore the land to the native savanna ecosystem. Soil and bark samples were processed through standard methods specifically for myxobacteria and numbers and types of species were recorded for both locations. Species were identified through morphology of fruiting bodies, SDS-PAGE of whole cell protein profiles, and 16S rRNA gene sequences. Statistical analyses were employed and suggested that significantly greater numbers and types of myxobacteria are present on the bark of the trees in the established oak-hickory forest than the bark of trees in the savanna restoration site, while little difference in numbers and types of species were observed between the soil samples of the two locations.

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Acknowledgments

Special thanks are extended to the Missouri Department of Natural Resources for letting us work on their land, Lisa Hooper and Jeffery Osborn of Truman State University who aided in tree species identification and the use of microscopes, Lawrence Shimkets of the University of Georgia for lending us reference strains, and Rolf Müller of GBF Gesellschaft für Biotechnologische Forschung mbH for giving us the primer sequences.

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Author notes

  1. R. Brock Neil

    Present address: Department of Microbiology, University of Iowa, College of Medicine, 3-403 Browen Science Building, 51 Newton Road, Iowa City, Iowa, 52242-1109, USA

  2. Keesoo Lee

    Present address: University of Missouri at Rolla, Center for Environmental Science, 1870 Miner Circle, Rolla, Missouri, 65401, USA

Authors and Affiliations

  1. Science Division, Truman State University, 100 E. Normal Street, Kirksville, Missouri, 63501, USA

    R. Brock Neil, Dan Hite, Michael I. Kelrick, Michael L. Lockhart & Keesoo Lee

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  1. R. Brock Neil
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  2. Dan Hite
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Correspondence to R. Brock Neil.

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Neil, R.B., Hite, D., Kelrick, M.I. et al. Myxobacterial Biodiversity in an Established Oak-Hickory Forest and a Savanna Restoration Site. Curr Microbiol 50, 88–95 (2005). https://doi.org/10.1007/s00284-004-4406-4

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