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A Suitable Xylella fastidiosa CVC Strain for Post-Genome Studies

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Abstract

The genome sequence of the pathogen Xylella fastidiosa Citrus Variegated Chlorosis (CVC) strain 9a5c has revealed many genes related to pathogenicity mechanisms and virulence determinants.

However, strain 9a5c is resistant to genetic transformation, impairing mutant production for the analysis of pathogenicity mechanisms and virulence determinants of this fastidious phytopathogen. By screening different strains, we found out that cloned strains J1a12, B111, and S11400, all isolated from citrus trees affected by CVC, are amenable to transformation, and J1a12 has been used as a model strain in a functional genomics program supported by FAPESP (São Paulo State Research Foundation). However, we have found that strain J1a12, unlike strains 9a5c and B111, was incapable of inducing CVC symptoms when inoculated in citrus plants. We have now determined that strain B111 is an appropriate candidate for post-genome studies of the CVC strain of X. fastidiosa.

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Acknowledgments

This work was supported by Fundecitrus and Fapesp grant-in-aid for X. fastidiosa Functional-genomics Project Research 19999/04340-1. We thank Joseph M. Bové for the critical reviewing of the manuscript. We also thank A. J. Ayres for helpful suggestions.

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Authors and Affiliations

  1. Fundo de Defesa da Citricultura (Fundecitrus), Av. Dr. Adhemar Pereira de Barros, 201, 14807-040, V. Melhado-Araraquara, SP, Brazil

    Diva do Carmo Teixeira, Sanvai Regina Prado Rocha, Mateus de Almeida Santos & Anelise Galdino Mariano

  2. United States Department of Agriculture, Agricultural Research Service, Fruit Laboratory, Beltsville, Maryland, USA

    Wen Bin Li

  3. Alellyx Applied Genomics, Rua James Clerck Maxwell 320, Techno Park, Km 104–Rod Anhanguera, 13480-060–, Campinas, SP, Brazil

    Patricia Brant Monteiro

Authors
  1. Diva do Carmo Teixeira
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  2. Sanvai Regina Prado Rocha
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  3. Mateus de Almeida Santos
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  4. Anelise Galdino Mariano
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  5. Wen Bin Li
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  6. Patricia Brant Monteiro
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Correspondence to Diva do Carmo Teixeira.

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Teixeira, D.d.C., Rocha, S.R.P., Santos, M.d. et al. A Suitable Xylella fastidiosa CVC Strain for Post-Genome Studies. Curr Microbiol 49, 396–399 (2004). https://doi.org/10.1007/s00284-004-4363-y

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  • DOI: https://doi.org/10.1007/s00284-004-4363-y

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