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Identification of Burkholderia andropogonis with a Repetitive Sequence BOX Element and PCR

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Abstract

Burkholderia andropogonis is the causal agent of bacterial leaf stripe in sorghum. Strict import quarantine regulations of numerous countries mandate a need for development of a rapid, reliable, and cost-effective diagnostic technique for the identification of B. andropogonis. Using a primer corresponding to the bacterial repetitive BOX element and PCR, we developed a DNA fingerprint which differentiated B. andropogonis from other phytobacterial pathogens.

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Acknowledgments

This research was supported by the Sorghum/Millet Collaborative Research Program (INTSORMIL) AID/DAN-1254-G-00-1022-00 and the Kansas Agricultural Experiment Station. This is Contribution No. 03-134-J from the Kansas Agricultural Experiment Station, Kansas State University, Manhattan.

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  1. Department of Plant Pathology, Throckmorton Hall, Kansas State University, Manhattan, KS, 66506-5502, USA

    Bruce A. Ramundo & Larry E. Claflin

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  1. Bruce A. Ramundo
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  2. Larry E. Claflin
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Correspondence to Larry E. Claflin.

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Ramundo, B.A., Claflin, L.E. Identification of Burkholderia andropogonis with a Repetitive Sequence BOX Element and PCR. Curr Microbiol 50, 52–56 (2005). https://doi.org/10.1007/s00284-004-4354-z

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  • DOI: https://doi.org/10.1007/s00284-004-4354-z

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