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Isolation and Characterization of Novel Strains of Pseudomonas aeruginosa and Serratia marcescens Possessing High Efficiency to Degrade Gasoline, Kerosene, Diesel Oil, and Lubricating Oil

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Abstract

Bacteria possessing high capacity to degrade gasoline, kerosene, diesel oil, and lubricating oil were screened from several areas of Hokkaido, Japan. Among isolates, two strains, WatG and HokM, which were identified as new strains of Pseudomonas aeruginosa and Serratia marcescens species, respectively, showed relatively high capacity and wide spectrum to degrade the hydrocarbons in gasoline, kerosene, diesel, and lubricating oil. About 90–95% of excess amount of total diesel oil and kerosene added to mineral salts media as a sole carbon source could be degraded by WatG within 2 and 3 weeks, respectively. The same amount of lubricating oil was 60% degraded within 2 weeks. Strain HokM was more capable than WatG in degrading aromatic compounds in gasoline. This strain could also degrade kerosene, diesel, and lubricating oil with a capacity of 50–60%. Thus, these two isolates have potential to be useful for bioremediation of sites highly contaminated with petroleum hydrocarbons.

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Notes

  1. Hydrocarbon with x carbon atoms is presented as C x , normal Aliphatic hydrocarbon with x carbon atoms is abbreviated as nC x .

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Acknowledgments

This work was partially supported by Northern Advancement Center for Science & Technology.

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Authors and Affiliations

  1. ROM Co. Ltd., Minami 4, Nishi 9, Chuo-ku, Sapporo, 064-0804, Japan

    Patcharaporn Wongsa, Makiko Tanaka, Akio Ueno & Mohammad Hasanuzzaman

  2. Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo, 060-0810, Japan

    Patcharaporn Wongsa, Akio Ueno, Mohammad Hasanuzzaman & Hidetoshi Okuyama

  3. Institute for Biological Resource and Function, National Institute of Advanced Industrial Science and Technology, Toyohira-ku, Sapporo, 062-8517, Japan

    Makiko Tanaka & Isao Yumoto

Authors
  1. Patcharaporn Wongsa
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  2. Makiko Tanaka
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  6. Hidetoshi Okuyama
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Correspondence to Hidetoshi Okuyama.

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Wongsa, P., Tanaka, M., Ueno, A. et al. Isolation and Characterization of Novel Strains of Pseudomonas aeruginosa and Serratia marcescens Possessing High Efficiency to Degrade Gasoline, Kerosene, Diesel Oil, and Lubricating Oil. Curr Microbiol 49, 415–422 (2004). https://doi.org/10.1007/s00284-004-4347-y

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  • DOI: https://doi.org/10.1007/s00284-004-4347-y

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