Current Microbiology

, Volume 49, Issue 2, pp 79–83 | Cite as

Effect of Different Temperature Downshifts on Protein Synthesis by Aeromonas hydrophila

  • Marlène Imbert
  • Frédérique Gancel

Abstract

The psychrotrophic bacterium Aeromonas hydrophila 7966 was subjected to cold shocks from 30°C to 20°C, 15°C, 10°C, or 5°C, or were incubated at low temperature to determine its adaptative response. The cell protein patterns analyzed by two-dimensional electrophoresis revealed that only a few proteins were underexpressed, whereas numerous new proteins appeared with the decrease of temperature, and some others were overexpressed. Among them, a few constituted cold shock proteins because they were transiently induced, whereas others belong to the acclimatation family proteins. Two cold shock proteins of 11 kDa were synthesized at low level because they were visualized only after radiolabeling or silver staining. Moreover, under our experimental conditions, no major cold shock protein of a molecular mass similar to that of E. coli (7.4 kDa) could be identified.

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Copyright information

© Springer-Verlag New York Inc. 2004

Authors and Affiliations

  • Marlène Imbert
    • 1
  • Frédérique Gancel
    • 1
  1. 1.Laboratoire de Microbiologie, Université des Sciences et Technologies de Lille, Bâtiment SN2, F-59655 Villeneuve d’Ascq CedexFrance

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