Abstract
Extracellular protein profiles were compared for broth-grown cultures of Burkholderia pseudomallei and its avirulent close relative Burkholderia thailandensis. A number of protein bands were present in the B. pseudomallei profile but absent or less abundant in the B. thailandensis profile. Four such prominent secreted proteins were identified by using N-terminal sequencing coupled to searches of the B. pseudomallei genome sequence database. The genes for two proteins with similarity to carbohydrate-binding proteins, and a further protein homologous to known bacterial collagenases, were present in both B. pseudomallei and B. thailandensis. The putative collagenase gene was cloned and expressed as a fusion protein in Escherichia coli. Cell lysates from Escherichia coli containing the recombinant protein exhibited detectable gelatinase and collagenase activities.
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Rainbow, ., Wilkinson, ., Sargent, . et al. Identification and Expression of a Burkholderia pseudomallei Collagenase in Escherichia coli . Curr Microbiol 48, 300–304 (2004). https://doi.org/10.1007/s00284-003-4192-4
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DOI: https://doi.org/10.1007/s00284-003-4192-4