Abstract
Escherichia coli K-12 lysogens of three different Shiga toxin 2 (Stx2)-encoding bacteriophages were examined for variability in their pulsed-field gel electrophoresis (PFGE) fragment patterns. The PFGE fragment patterns could be classified into three types (i.e., PFGE types B, C, and D). For the PFGE type D, a 255-kbp fragment present in the original K-12 strain was apparently shifted by the size of Stx 2-encoding phage genomic DNA (ca. 65 kbp) to the position at 320 kbp. In contrast, the types B and C showed the above fragment shift plus further 6- and 10-fragment differences, respectively, from the original K-12 strain. The evidence suggests that even a single genetic event like lysogeny can cause marked genotypic modification of the host strain.
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Received: 21 June 2002 / Accepted: 5 July 2002
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Iguchi, A., Osawa, R., Kawano, J. et al. Effects of Lysogeny of Shiga Toxin 2-Encoding Bacteriophages on Pulsed-Field Gel Electrophoresis Fragment Pattern of Escherichia coli K-12. Curr Microbiol 46, 0224–0227 (2003). https://doi.org/10.1007/s00284-002-3900-9
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DOI: https://doi.org/10.1007/s00284-002-3900-9