Abstract
Scedosporium prolificans mutants lacking the ability to synthesize melanin were selected after ultraviolet light (UV) irradiation. UV exposure of S. prolificans conidia resulted in a high frequency of melanin-deficient (mel−) mutants. Stable and non-stable morphological variants were found in the population: reversion of the mutant phenotype was always to the wild-type phenotype. Based on their morphological differences, these variants were classified into five different groups that were phenotypically characterized. The mel− mutants plus the wild-type strain were examined for in vitro susceptibility to antifungal agents with different and/or the same mechanism of action. There was no apparent difference in minimum inhibitory concentrations when comparing the wild-type and the mel− mutants. Therefore, melanin does not appear to confer protection against the more important antifungal agents in S. prolificans.
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Received: 30 April 2002 / Accepted: 10 July 2002
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Ruiz-Díez, B., Martínez-Suárez, J. Isolation, Characterization, and Antifungal Susceptibility of Melanin-Deficient Mutants of Scedosporium prolificans . Curr Microbiol 46, 0228–0232 (2003). https://doi.org/10.1007/s00284-002-3858-7
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DOI: https://doi.org/10.1007/s00284-002-3858-7