Abstract
Porphyromonas gingivalis seems to perturb the cytokine network to maintain periodontal disease. Although endothelial cells are a leading source of interleukin 8 (IL-8) and monocyte chemotactic protein 1 (MCP-1), the effect of P. gingivalis on the cells remains unclear. We used reverse transcription-PCR (RT-PCR) to assess levels of IL-8 and MCP-1 mRNA and enzyme-linked immunosorbent assays (ELISA) to evaluate their protein production by human umbilical vein endothelial cells (HUVEC) challenged with P. gingivalis. IL-8 and MCP-1 protein levels decreased in response to challenge with P. gingivalis, and N-a-p-tosyl-L-lysine chloromethylketone (TLCK) prevented the degradation of these chemokines. Furthermore, the bacteria upregulated expression of the mRNAs of these chemokines. Our results indicate that P. gingivalis proteases degraded IL-8 and MCP-1. Degradation of chemokines secreted from endothelial cells may decrease the recruitment of leukocytes and their migration through the endothelium, thus contributing to a delay in the host immune defense mechanism.
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Received: 1 February 2002 / Accepted: 30 April 2002
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Kobayashi-Sakamoto, M., Isogai, E. & Hirose, K. Porphyromonas gingivalis Modulates the Production of Interleukin 8 and Monocyte Chemotactic Protein 1 in Human Vascular Endothelial Cells. Curr Microbiol 46, 0109–0114 (2003). https://doi.org/10.1007/s00284-002-3782-x
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DOI: https://doi.org/10.1007/s00284-002-3782-x