Skip to main content
SpringerLink
Log in

Cloning of a Gene Encoding Acetate Kinase from Methanosarcina mazei 2-P Isolated from a Japanese Paddy Field Soil

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

The ack gene encoding acetate kinase from the mesophilic Methanosarcina mazei 2-P, isolated from a paddy field soil in Japan, was cloned, sequenced, and functionally expressed in Escherichia coli. The terminal region of the putative pta gene, probably encoding phosphotransacetylase, was found upstream of the ack gene. The deduced amino acid sequence of the acetate kinase is 86.5% identical to that of the Methanosarcina thermophila acetate kinase. The activity of the His6-tagged acetate kinase purified from E. coli JM109 was optimal at 35°C.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Japan, , , , , , JP

    Akio Tonouchi, Yousuke Nishizaki, Hiromi Tohyama & Kiyoshi Takeda

Authors
  1. Akio Tonouchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yousuke Nishizaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hiromi Tohyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kiyoshi Takeda
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Received: 28 January 2002 / Accepted: 27 February 2002

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tonouchi, A., Nishizaki, Y., Tohyama, H. et al. Cloning of a Gene Encoding Acetate Kinase from Methanosarcina mazei 2-P Isolated from a Japanese Paddy Field Soil. Curr Microbiol 45, 0390–0393 (2002). https://doi.org/10.1007/s00284-002-3779-5

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-002-3779-5

Keywords

Search

Navigation