Abstract
A library of insertional mutants of Enterococcus faecalis was constructed; it allowed the isolation and the characterization of 10 mutants affected in resistance to bile salts. Insertion loci of two mutants corresponded to genes of unknown function, while the amino acid sequences deduced from the other loci were homologous to proteins related to DNA repair, oxidative response, transcriptional regulation, dGTP hydrolysis, membrane composition, or cell wall synthesis. Further characterization of one mutant revealed that the insertion within the E. faecalis sagA gene led to a decrease of the resistance towards numerous independent physicochemical stresses, to modifications of the cell wall integrity, and to perturbations of cell division with septation anomalies.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 1 November 2001 / Accepted: 27 March 2002
Rights and permissions
About this article
Cite this article
Breton, Y., Mazé, A., Hartke, A. et al. Isolation and Characterization of Bile Salts-Sensitive Mutants of Enterococcus faecalis . Curr Microbiol 45, 0434–0439 (2002). https://doi.org/10.1007/s00284-002-3714-9
Issue Date:
DOI: https://doi.org/10.1007/s00284-002-3714-9