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Oligo-microarray analysis reveals the role of cyclophilin A in drug resistance

Cancer Chemotherapy and Pharmacology volume 61, pages 459–469 (2008)Cite this article

Abstract

Cyclophilin A (CYPA) belongs to peptidyl prolyl isomerases (PPIases), which catalyze the cis/trans isomerization of prolyl peptide bonds in cellular communication. CYPA has been implicated in several pathological processes, including cancer, inflammatory diseases, and HIV-1 infection. Up-regulation of CYPA has been found to be a common phenomenon in several tumor types, including in hepatocellular carcinoma (HCC). However, the role of CYPA in tumor cells remains unknown. We generated a stable SK-Hep1 cell line and studied the CYPA regulated genes at the transcriptome level. The microarray results reveal that CYPA can up-regulate the expression of many cytokine and drug resistance related genes. Furthermore, we showed that the elevated CYPA expression contributes to drug resistance. We postulate that the over-expression of CYPA in tumors may play a role in clinical resistance to chemotherapy.

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Acknowledgments

This work was supported by the Graduate Innovation Foundation of Fudan University (No. EYH1322021).

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  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, 200433, People’s Republic of China

    Shuai Chen, Mingjun Zhang, Honghui Ma, Hexige Saiyin, Suqin Shen, Jiajie Xi, Bo Wan & Long Yu

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  1. Shuai Chen
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Correspondence to Long Yu.

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280_2007_491_MOESM1_ESM.doc

The specificity of rabbit multiclonal antibody of CypA was evaluated by western blot. As shown in the figure, the specificity is high and no other band detected (DOC 107 kb)

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Chen, S., Zhang, M., Ma, H. et al. Oligo-microarray analysis reveals the role of cyclophilin A in drug resistance. Cancer Chemother Pharmacol 61, 459–469 (2008). https://doi.org/10.1007/s00280-007-0491-y

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  • DOI: https://doi.org/10.1007/s00280-007-0491-y

Keywords

  • Oligo-microarray
  • Peptidyl–prolyl isomerase (PPIase)
  • Cyclophilin A (CYPA/PPIA)
  • Hepatocellular carcinoma (HCC)
  • Multi-drug resistant protien (MRP)