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Circulating miR-455-3p, miR-5787, and miR-548a-3p as potential noninvasive biomarkers in the diagnosis of acute graft-versus-host disease: a validation study

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Abstract

Currently, acute graft-versus-host disease (aGVHD) diagnosis is based on clinical features and pathological findings. Until now, there is no non-invasive diagnostic test for aGVHD. MicroRNAs may act as promising predictive, diagnostic, or prognostic biomarkers for aGVHD. The purpose of the current study was to validate circulating microRNAs as diagnostic biomarkers to assist clinicians in promptly diagnosing aGVHD, so that treatment can be initiated earlier. In the present study, we evaluated six microRNAs (miR-455-3p, miR-5787, miR-6729-5p, miR-6776-5p, miR-548a-3p, and miR-6732-5p) selected from miRNA array data in 40 aGVHD patients compared to 40 non-GVHD patients with RT-qPCR. Target genes of differentially expressed microRNAs (DEMs) were predicted using Targetscan, miRanda, miRDB, miRWalk, PICTAR5, miRmap, DIANA, and miRTarBase algorithms, and their functions were analyzed using EnrichNet, Metascape, and DIANA-miRPath databases. The expressions of plasma miR-455-3p and miR-5787 were significantly downregulated, whereas miR-548a-3p was significantly upregulated in aGVHD patients compared to non-GVHD patients. Moreover, DEMs showed potentially high diagnostic accuracy for aGVHD. In silico analysis of DEMs provided valuable information on the role of DEMs in GVHD, immune regulation, and inflammatory response. Our study suggested that miR-455-3p, miR-5787, and miR-548a-3p could be used as potential noninvasive biomarkers in the diagnosis of aGVHD in addition to possible therapeutic targets in aGVHD.

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Funding

This study was supported by grants from Dr. Marjan Yaghmaie (Grant# 95- 04–36-33688) and Dr. Mohammad Ahmadvand from Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran, and Student Research Committee (Proposal No. 971837), Mashhad University of Medical Sciences, Mashhad, Iran.

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  1. Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Jamshid Motaei, Seied Asadollah Mousavi, Kamran Alimoghadam, Ardeshir Ghavamzadeh, Saba Manoochehrabadi, Mohammad Ahmadvand & Marjan Yaghmaie

  2. Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran

    Jamshid Motaei

  3. Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Jamshid Motaei & Mohammad Amin Kerachian

  4. Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Jamshid Motaei & Mohammad Amin Kerachian

  5. Cancer Genetics Research Unit, Reza Radiotherapy and Oncology Center, Mashhad, Iran

    Mohammad Amin Kerachian

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Motaei, J., Kerachian, M.A., Mousavi, S.A. et al. Circulating miR-455-3p, miR-5787, and miR-548a-3p as potential noninvasive biomarkers in the diagnosis of acute graft-versus-host disease: a validation study. Ann Hematol 100, 2621–2631 (2021). https://doi.org/10.1007/s00277-021-04573-1

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