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Phenotypic characterization of macrophages in the BMB sample of human acute leukemia

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Abstract

Macrophages within tissues display a strong plastic ability in respond to environmental cues in both physiologic influences and disease. However, the macrophage phenotype and its distribution in the bone marrow biopsies (BMB) samples of human acute leukemia (AL) remain poorly understood. In this study, 97 BMB samples of patients with acute leukemia and 30 iron-deficiency anemias (IDA) as control group were evaluated with immunohistochemistry. In comparison with controls, the counts of CD68+, CD163+, and CD206+macrophages were remarkably increased in BMB samples of acute leukemia (P < 0.01), as well as their infiltration density was roaring up-regulation (P < 0.01). The expression levels of CD68+, CD163+, and CD206+macrophages were decreased in patients with complete remission, but there still existed statistically significant contrast to the control group (P < 0.01). The ratios of the CD163-positive cells or CD206-positive cells to CD68-positive cells were most prevalent in the BMB samples of human acute leukemia compared with the control group (P < 0.01), which support that macrophages were polarized to M2 macrophages.

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Acknowledgments

We gratefully acknowledge the kind cooperation of the acute leukemia patients and the iron-deficiency anemia (IDA) patients.

Funding

This work was supported by grants from “the National Science Funding of China,” Grants: No.31460298, the Foundation Dr. Sa YL and “the Basic Research on Application of Joint Special Funding of Science and Technology Department of Yunnan Province-Kunming Medical University,” Grants: No.2014FZ070, the Foundation Mr. Song JX.

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Authors and Affiliations

  1. Department of Clinical Laboratory, The First People’s Hospital of Yunnan Province, Kunming, 650032, Yunnan, China

    Jian-Xin Song, Ting Dong & Yi-Fei Tang

  2. Institute of Clinical and Basic Medical Sciences (Yunnan Provincial Key Laboratory of Clinical Virology; Key Laboratory for Birth Defects and Genetic Diseases), The First People’s Hospital of Yunnan Province, Kunming, 650032, Yunnan, China

    Jian-Xin Song, Rui-Wei Li, Juan-Juan Zhang & Ya-Lian Sa

  3. Department of Hematology, The First People’s Hospital of Yunnan Province, Kunming, 650032, Yunnan, China

    Yan Wen

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  1. Jian-Xin Song
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Contributions

Song JX carried out major experiments and partially participated in its design. Wen Y collected the BMB sample and partially performed the experiments. Li RW performed the experiments and analyzed the data. Dong T carried out the experiments. Tang YF contributes to data interpretation. Zhang JJ contributes in editing the study. Sa YL designed the project, drafted the manuscript, edited the manuscript, and submitted the manuscript. Both authors read and approved the final manuscript.

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Correspondence to Ya-Lian Sa.

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The authors declare that they have no conflict of interest.

Ethical approval

This study was approved by the Medical Ethics Committee of Yunnan Provincial First People’s Hospital. All procedures performed in the study were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Written informed consent was obtained from patients to authorize their participation in the study.

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Song, JX., Wen, Y., Li, RW. et al. Phenotypic characterization of macrophages in the BMB sample of human acute leukemia. Ann Hematol 99, 539–547 (2020). https://doi.org/10.1007/s00277-020-03912-y

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  • DOI: https://doi.org/10.1007/s00277-020-03912-y

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