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The prevalence of chromosomal aberrations associated with myelodysplastic syndromes in China

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Abstract

This study aims to investigate the prevalence and distribution of diverse chromosomal aberrations associated with myelodysplastic syndromes (MDS) in China. Bone marrow samples were collected from multiple cities in China. Metaphase cytogenetic (MC) analysis and fluorescence in situ hybridization (FISH) were initially used to test chromosomal lesions. Affymetrix CytoScan 750 K genechip platform performed a genome-wide detection of chromosomal aberrations. Chromosomal gain was identified in 76 patients; the most prevalent was trisomy 8(17.9 %). New chromosomal gain was detected on chromosome 9, 19p, and X. Chromosomal loss was detected in 101 patients. The most frequent was loss 5q (21.0 %). Some loss and gain were not identified by MC or FISH but identified by genechip. UPD was solely identified by genechip in 51 patients; the most prevalent were UPD 7q (4.94 %) and UPD 17p (4.32 %). Furthermore, complex chromosomal aberrations were detected in 56 patients. In conclusion, Affymetrix CytoScan 750 K genechip was more precise than MC and FISH in detection of cryptic chromosomal aberrations relevant to MDS. Analysis of the prevalence and distribution of diverse chromosomal aberrations in China may improve strategies for MDS diagnosis and therapies.

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Acknowledgments

This study was sponsored by the National Natural Science Foundation of China (No. 81372407).

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    Authors and Affiliations

    1. Department of Oncology I, Renmin Hospital, Wuhan University, Road No. 9, Wuhan, China

      Qinyong Hu, Yuxin Chu, Qibin Song & Yi Yao

    2. Department of Molecular Diagnosis, Kindstar Global, Biolake B3-1, Wuhan, China

      Weihong Yang & Shiang Huang

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    1. Qinyong Hu
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    2. Yuxin Chu
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    3. Qibin Song
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    Correspondence to Qibin Song or Shiang Huang.

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    The authors declare that they have no conflict of interest.

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    Informed consent was signed for each patient according to protocols permitted by Institutional Ethics Committee of Wuhan University.

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    Qinyong Hu and Yuxin Chu contributed equally to this work.

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    Hu, Q., Chu, Y., Song, Q. et al. The prevalence of chromosomal aberrations associated with myelodysplastic syndromes in China. Ann Hematol 95, 1241–1248 (2016). https://doi.org/10.1007/s00277-016-2698-7

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    • DOI: https://doi.org/10.1007/s00277-016-2698-7

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