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Antiplatelet antibodies detected by the MAIPA assay in newly diagnosed immune thrombocytopenia are associated with chronic outcome and higher risk of bleeding

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Abstract

Immune thrombocytopenia (ITP) results in part from the presence of platelet antibodies, which can be demonstrated by the Monoclonal Antibody-Specific Immobilization of Platelet Antigens (MAIPA) assay. The aim of our study was to correlate the presence of antiplatelet autoantibodies and the natural history of ITP. We performed a retrospective, single-center study of 108 adults with newly diagnosed ITP who had indirect MAIPA assay performed at disease onset. Chronic ITP was defined by the presence of thrombocytopenia after 1 year. Bleeding diathesis was evaluated with a bleeding score. At baseline, patients with a positive indirect MAIPA have a greater bleeding score than patients with negative MAIPA assay [median (interquartile) = 8 (6–12) vs 2 (0–6), p = 0.002]. Patients with a positive indirect MAIPA also had a higher rate of chronic ITP (92.9 vs 68.7 %, p = 0.06). In multivariate analysis, a positive indirect MAIPA result and a platelet count at onset ≥10 × 109/L remained independently associated with chronic ITP [adjusted OR (aOR) = 8.01; 95 % confidence interval (CI), 0.98–66.6; p = 0.05 and aOR = 3.09; 95 % CI, 1.18–8.10; p = 0.02, respectively]. Furthermore, when we analyzed together the results of direct (n = 41) and indirect MAIPA, the same results were observed. Thus, indirect MAIPA positivity at disease onset is associated with more severe hemorrhage and predicts a chronic course in adult ITP patients. MAIPA assay could be useful in the management of ITP patients when it is performed at diagnosis.

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Acknowledgment

The authors would like to gratefully thank Aurélie Le Thuaut for her technical help in statistical analysis. DG, BG and PB designed the study. BG, PB, MK, MM enrolled the patients. DG and LL recorded the data of the study. FCP and FRT performed the statistical analysis. KL, LC and PB performed MAIPA test. DG, FCP, MK, MM, BG and PB wrote the manuscript.

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Authors and Affiliations

  1. Service de Médecine interne, Centre de référence des cytopénies auto-immunes, hôpital Henri Mondor, AP-HP, Université Paris Est, Créteil, 94010, France

    David Grimaldi, Laure Croisille, Ketty Lee, Laetitia Languille, Medhi Khellaf, Marc Michel, Bertrand Godeau & Philippe Bierling

  2. Université Paris Est, LIC EA 4393, Créteil, 94010, France

    Florence Canouï-Poitrine & Françoise Roudot-Thoraval

  3. AP-HP, Service de santé publique, Hôpital Henri Mondor, Créteil, 94010, France

    Florence Canouï-Poitrine & Françoise Roudot-Thoraval

  4. Etablissement Francais du Sang, Hopital Henri Mondor, Creteil, 94010, France

    Laure Croisille, Ketty Lee & Philippe Bierling

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  1. David Grimaldi
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  2. Florence Canouï-Poitrine
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  3. Laure Croisille
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  4. Ketty Lee
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  6. Laetitia Languille
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  7. Medhi Khellaf
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  8. Marc Michel
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  9. Bertrand Godeau
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  10. Philippe Bierling
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Correspondence to Philippe Bierling.

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Grimaldi, D., Canouï-Poitrine, F., Croisille, L. et al. Antiplatelet antibodies detected by the MAIPA assay in newly diagnosed immune thrombocytopenia are associated with chronic outcome and higher risk of bleeding. Ann Hematol 93, 309–315 (2014). https://doi.org/10.1007/s00277-013-1855-5

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  • DOI: https://doi.org/10.1007/s00277-013-1855-5

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