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Impact of NOD2 polymorphisms on infectious complications following chemotherapy in patients with acute myeloid leukaemia

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Abstract

We sought to investigate the relationship between polymorphisms of the NOD2 gene and infectious complications following intensive induction chemotherapy in patients with acute myeloid leukaemia (AML). We hypothesised that single nucleotide polymorphisms (SNPs) of the NOD2 gene are associated with a higher rate of infections during the phase of severe neutropenia. In 131 AML patients receiving induction therapy, the presence of the three most frequent polymorphisms of NOD2 (Arg702Trp, Gly908Arg, Leu1007fsinsC) was analysed. SNP analyses by means of genomic PCR incorporating fluorescence-labelled probes with characteristic melting curves were performed using the LightCycler platform. Our data suggest a significantly lower probability of mucositis or enteritis in AML patients lacking any of the three evaluated NOD2 polymorphisms. Furthermore, bloodstream cultures of AML patients carrying either a missense or a frameshift mutation of NOD2 were significantly more frequently tested positive concerning Streptococcus spp. In contrast, the presence of NOD2 polymorphisms had no impact on such important infectious complications as systemic inflammatory response syndrome or sepsis, the rate of central venous catheter infections or the incidence of pneumonia including fungal infections. Our data represent one of the first reports investigating the impact of polymorphisms of the innate immune system on infectious complications in patients with neutropenia following chemotherapy. A correlation between NOD2 polymorphisms and infectious events in AML patients is demonstrated.

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Acknowledgments

This work was supported by a grant from the Deutsche José Carreras Leukämie-Stiftung (Project 11/03).

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Abteilung Hämatologie/Internistische Onkologie, Klinik für Innere Medizin II, Jena University Hospital, Erlanger Allee 101, 07740, Jena, Germany

    Olaposi Yomade, Bärbel Spies-Weisshart, Ulf Schnetzke, Andreas Hochhaus & Sebastian Scholl

  2. Institut für Humangenetik und Anthropologie, Jena University Hospital, Jena, Germany

    Anita Glaser

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  1. Olaposi Yomade
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  2. Bärbel Spies-Weisshart
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  4. Ulf Schnetzke
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  5. Andreas Hochhaus
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  6. Sebastian Scholl
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Correspondence to Sebastian Scholl.

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Yomade, O., Spies-Weisshart, B., Glaser, A. et al. Impact of NOD2 polymorphisms on infectious complications following chemotherapy in patients with acute myeloid leukaemia. Ann Hematol 92, 1071–1077 (2013). https://doi.org/10.1007/s00277-013-1734-0

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  • DOI: https://doi.org/10.1007/s00277-013-1734-0

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