Abstract
MicroRNAs (miRNAs) are negative regulators of gene expression that play an important role in hematopoiesis. Thalassemia, a defective globin synthesis leading to precipitate of excess unbound globins in red blood cell precursors, results in defective erythroid precursors and ineffective erythropoiesis. Expression pattern of miR-451, an erythroid-specific miRNA, was analyzed during differentiation of erythroid progenitors derived from normal and thalassemic peripheral blood CD34-positive cells, after 14 days of culture. A biphasic expression with transient up-regulation of miRNA-451 on day 3 of cultures was observed during thalassemic erythroid differentiation. In contrast, the expression pattern of the miR-451 in erythroid cells obtained from the other extravascular hemolytic anemia, i.e., hereditary spherocytosis patients showed no transient up-regulation of miR-451 on day 3 of cultures. Our results suggest that early erythroid progenitors in β-thalassemia have a dysregulated miRNA-451 expression program, and analysis of microRNA is a relevant approach to determine abnormalities of erythropoiesis.
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Acknowledgments
This study was supported in part by National Science and Technology Development Agency, Thailand (NSTDA), Mahidol University Research Grant, Japan Society for the Promotion of Science (JSPS), and the National Research Council of Thailand (NRCT). This work was supported in part by the Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Exploratory Research, 2009, 21659240.
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SS contributed to the study design, interpretation of the data, drafting, and editing of the manuscript. SM and TP performed the research and analyzed the data. YA, PW, and SF were responsible for study design and specimen collection. TU contributed to the design, concept of the study, and editing of the manuscript. Final approval of the version to be published was made by all authors.
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Svasti, S., Masaki, S., Penglong, T. et al. Expression of microRNA-451 in normal and thalassemic erythropoiesis. Ann Hematol 89, 953–958 (2010). https://doi.org/10.1007/s00277-010-0980-7
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DOI: https://doi.org/10.1007/s00277-010-0980-7