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Prognostic Significance of MicroRNA-16 Expression in Human Colorectal Cancer

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World Journal of Surgery Aims and scope Submit manuscript

Abstract

Background

MicroRNAs (miRNAs), small noncoding RNAs, have been reported to be highly involved in the formation and progression of all types of human cancer including colorectal cancer (CRC). Therefore, miRNAs are also potential prognostic biomarkers in CRC patients. The aim of this study was to detect the expression of miR-16 in human CRC tissues and investigate its clinicopathologic or prognostic significance.

Methods

TaqMan quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay was performed to determine the expression of miR-16 in 143 primary CRC tissues and 18 corresponding normal colonic mucosa from patients who had undergone surgery. The association of miR-16 expression with clinicopathologic features of CRC patients was statistically analyzed. Kaplan–Meier analyses were used to assess patient survival. Univariate and multivariate Cox analyses were performed.

Results

The relative level of miR-16 in 18 CRC tissues was significantly lower than that in corresponding normal colonic mucosa (p < 0.001). Statistical analyses revealed that the status of miR-16 expression was closely associated with tumor differentiation, lymph node metastasis, L category, V category, TNM stage, and tumor recurrence of CRC (p = 0.001, 0.003, 0.001, 0.005, 0.003, and 0.017, respectively). Kaplan–Meier analyses indicated that patients with low-miR-16 had lower 5-year overall survival than those with high-miR-16 (31.2 vs. 58.3 %; p = 0.0012). Multivariate Cox regression analyses indicated that the status of miR-16 expression might be an independent prognostic factor for CRC patients (hazard ratio 1.67; 95 % confidence interval 1.22–2.54; p = 0.018).

Conclusions

Down-regulation of miR-16 plays critical roles in CRC progression. Low miR-16 expression is an independent factor predicting a poor prognosis for CRC patients.

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Acknowledgments

The authors thank Professor Ming Zhang for expert advice on pathology. We also thank everyone in the Department of Pathology for their technical assistance.

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Authors and Affiliations

  1. Department of Oncology, The Third Affiliated Hospital, Nanjing University of Traditional Chinese Medicine, No. 1 Jinling Road, Nanjing, Jiangsu, 210029, China

    Jun Qian, Bin Jiang, Min Li, Juan Chen & Mingzhi Fang

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  1. Jun Qian
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  2. Bin Jiang
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  3. Min Li
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  4. Juan Chen
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  5. Mingzhi Fang
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Corresponding author

Correspondence to Mingzhi Fang.

Electronic supplementary material

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268_2013_2205_MOESM1_ESM.tif

Supplementary Fig. S1 A ROC curve for survival prediction was plotted to identify the optimum cutoff point for miR-16 in CRC, which was 0.52. The area under the curve for miR-16 was 70.5 % (95 % confidence interval 0.678–0.768) with sensitivity of 63.6 % and specificity of 74.4 % (p = 0.012) Supplementary material 1 (TIFF 14 kb)

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Qian, J., Jiang, B., Li, M. et al. Prognostic Significance of MicroRNA-16 Expression in Human Colorectal Cancer. World J Surg 37, 2944–2949 (2013). https://doi.org/10.1007/s00268-013-2205-4

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  • DOI: https://doi.org/10.1007/s00268-013-2205-4

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